摘要
本实验选用兔61只,于双侧膝关节股骨髁部造成4×8mm^2的全层关节软骨缺损,取自体骨膜固定于缺损处,左膝骨膜生发层朝向骨面,右膝朝向关节腔。对照兔不作移植。术后不固定。分别于术后第1、2、4、8、12和16周取材,从组织学、组织化学、电镜及放射自显影等方面对新生组织进行研究。结果表明:(1)术后4周,由骨膜形成的新生组织已具有幼稚软骨的特征。8周后基本与正常关节软骨一致。对照组基本无软骨组织形成;(2)移植骨膜的生发层朝向关节腔者优于朝向骨面者;(3)移植骨膜形成的新生软骨具有与正常关节软骨基本一致的吸收35S形成"硫结节"的代谢特点。
Sixty-one rabbits were used in this study. A full-thickness defect of size 4×8mm^2 was created in the femoral articular cartilage on both sides. In the grafting rabbit. the periosteum was transplanted to the defect and sutured to the synovial membrane with the cambium layer facing the spongiosa on the left,while on the right side the cambium layer facing the articular cavity. Postoperatively, the animals were sacrificed at intervals of 1,2,4,8,12 and 16 weeks and the newly formed tissue in the defect was investigated histologically, histochemically, electron microscope and autoradiography with ^(35)S. The results are as follows;(1) At 4th week, the newly formed tissue originating from the grafting periosteum had the speciality of young cartilage. After 8 weeks, it resembled the normal articular cartilage; (2) Although the periosteum could form byline-like cartilage regardless of whether it was reversed or not, the quality of cartilage produced by the periosteum of cambium layer facing articular cavity was better than that facig spongiosa with a statistical significance;(3) The newly formed tissue that had formed from the grafting periosteum had the same ability in metabolism to concentrate the ^(35)S as the normal cartilage.
出处
《创伤杂志》
CSCD
北大核心
1989年第4期207-210,258,共4页
