摘要
目的 调查 3～ 4岁儿童口腔中变形链球菌的定植情况及其和母亲的关系。方法 选择 5 0名 3～ 4岁儿童和他们的母亲 ,依据儿童口腔龋齿情况分成三组。分离牙菌斑和唾液样本中的变链菌 ,计算检出率。同时以AP- PCR检测分离株的基因型。结果 5 0名儿童有 37人 (74 % )检出变链菌 ,其中 2 4名无龋儿童中 11名 (4 5 .8% )检出变链菌 ,2 6名有龋儿童中 ,全部检出变链菌 ,两组之间差别有显著性 (P<0 .0 1)。母亲唾液和菌斑变链菌的检出率分别为 32 %和 5 6 % ,差别显著 (P<0 .0 1)。 AP- PCR指纹显示 4 3.2 %的儿童变链菌基因型和其母亲一致。结论 儿童龋齿与变链菌的早期获得密切相关。母亲依然是 3～ 4岁儿童口腔中变链菌的主要来源。
Objective The aim of this study was to investigate the prevalence of Mutans streptococci (MS) in children of 3-4 years and thus reveal the relations hip between the children's acquisition of MS and their mothers' pathogen. Methods Fifty mother-child pairs were selected, examined and divided into three groups according to the children's caries. MS in plaque and mothers' salivary samples were detected by MSB medium. Then 200 MS strains from 20 mothers-children were analyzed by AP-PCR. Results Acquisition of MS was identified in 37 of 50 children (74%), including 11 of 24 caries-free children and all 26 children with caries. The difference was significant (P<0.01).Genotypes showed that 16 of 37 children(43.2%) had the same fingerprint as their mothers'. The level of MS identified in mothers' salivary sample was lower than that in mothers' plaque sample (32% and 56%). Conclusion These results suggested that caries in children of 3-4 years are closely related with MS acquisition. Mothers are still their important source of MS. The sensitivity of mothers' salivery samples is much lower than that of plaque samples in studying the transmission of MS.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2004年第6期818-820,共3页
Journal of Sichuan University(Medical Sciences)
基金
国家自然科学基金 (批准号 3 0 1710 13 )
安徽省教育厅资助项目资助
关键词
变形链球菌
定植
传播
Mutans streptococci Colonization Transmission
