摘要
Pattern recognition methods were applied to the analysis of 600 MHz H NMR spectra of urine from rats dosed 1 with compounds that induced organ-specific damage in the liver and kidney. Male Wistar rats were separated into groups (n=4) and each was treated with one of following compounds: HgCl2, CCl4, Lu(NO3)3 and Changle (a kind of rare earth complex mixed with La, Ce, Pr and Nd). Urine samples from the rats dosed with HgCl2, CCl4 and Lu(NO3)3 were collected over a 24 h time course and the samples from the rats administrated with Changle were gained after 3 months. These samples were measured by 600 MHz NMR spectroscopy. Each spectrum was data-processed to provide 223 intensity-related descriptors of spectra. Urine spectral data corresponding to the time intervals, 0—8 h (HgCl2 and CCl4), 4—8 (Lu(NO3)3) h and 90 d (Changle) were analyzed using principal compo- nent analysis (PCA). Successful classification of the toxicity and biochemical effects of Lu(NO3)3 was achieved.
Pattern recognition methods were applied to the analysis of 600 MHz H NMR spectra of urine from rats dosed 1 with compounds that induced organ-specific damage in the liver and kidney. Male Wistar rats were separated into groups (n=4) and each was treated with one of following compounds: HgCl2, CCl4, Lu(NO3)3 and Changle (a kind of rare earth complex mixed with La, Ce, Pr and Nd). Urine samples from the rats dosed with HgCl2, CCl4 and Lu(NO3)3 were collected over a 24 h time course and the samples from the rats administrated with Changle were gained after 3 months. These samples were measured by 600 MHz NMR spectroscopy. Each spectrum was data-processed to provide 223 intensity-related descriptors of spectra. Urine spectral data corresponding to the time intervals, 0—8 h (HgCl2 and CCl4), 4—8 (Lu(NO3)3) h and 90 d (Changle) were analyzed using principal compo- nent analysis (PCA). Successful classification of the toxicity and biochemical effects of Lu(NO3)3 was achieved.
基金
Project supported by the National Natural Science Foundation of China (No. 29890280).
