摘要
以大豆品种小黑豆的子叶节为外檀体,通过附加外源的6-BA刺激子叶节,使其膨大增粗.切下子叶节培养,结果每个子叶节最高能分化出11颗正常的苗。利用含非致瘤性Ti质粒载体的根癌农杆菌感染巳膨大的子叶节,可得到转基因的再生绿苗.该质粒载体带有一个npt-Ⅱ基因(新霉素磷酸基转移酶Ⅱ基因)和一个胭脂碱合成酶基因.对再生植株的胭脂碱测定。NPT-Ⅱ酶活性检测及DNA分子杂交试验表明,外源基因巳导入大豆子叶节细胞,并能在植株水平表达出相应的性状。
With cotyledonary nodes of the soybean cv. Xiaoheidou as explants, the effects of hormone(6-BA)on the
capacity of differentiation and plant regeneration were studied. By stimulating cotyledonary nodes using 6-BA.
as many as 11 morphologically normal plants from multiple buds of each cotyledonary node were obtained.
Cotyledonary nodes of soybean could be infected by Agrobacterium tumefaciens harboring a non-oncogenic Ti
plasmid derived vector which containing a npt-Ⅱ gene and a nopaline synthetase gene. Trensgenic green
plantlets were obtained. Successful genetic transformation was confirmed by nopaline assay, npt-Ⅱ enzyme
activity assay and DNA hybridization.
出处
《科技通报》
1993年第4期218-220,225,共4页
Bulletin of Science and Technology
关键词
大豆
子叶节
根瘤农杆菌
转化
soybean
cotyledonary node
agrobacterium tumefaciens
transformation
