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提取大白鼠脂肪干细胞的初步研究 被引量：9

Stem cell from murine adipose tissue
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摘要 目的探讨自脂肪中分离干细胞的方法。方法取大白鼠皮下脂肪组织,经消化、离心、去除红细胞等步骤,培养,间日换液。结果出现呈梭形成纤维细胞样生长的细胞,细胞增殖旺盛。结论可自大鼠皮下脂肪获得多能干细胞。 Objective: To discuss the method of isolating stem cell from adipose tissue. Methods: The subcutaneous adipose tissue from murine was digested, centrifugated, deodrythrocyted, etc and cultivated with DMEM/F12. The cultivation was exchanged once 2 days. Results: The cell spread fusiform shape, and proliferation with rapid rate. Conclusion: The cell from murine subcutaneous adipose tissue may be multipotential stem cell.
作者 邓展生 鞠洪斌 沈民仁 许宇霞 李宝军 张胜利
机构地区 中南大学湘雅医院骨科
出处 《中国医学工程》 2004年第5期46-47,共2页 China Medical Engineering
关键词 提取方法 大白鼠 脂肪干细胞 梭形成纤维细胞样生长 皮下脂肪 tissue engineering adipose tissue isolation stem cell
分类号 Q813 [生物学—生物工程]
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参考文献8

  • 1[1]JM Gimblel, F Guilak. Adipose-derived adult stem cells: isolation, characterization, and differentiation potential[J]. Cytotherapy,2003, 5(5): 362-369.
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共引文献20

同被引文献78

引证文献9

二级引证文献29

中国医学工程
2004年 第5期

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