摘要
目的对SLE患者外周血中单核细胞诱导,培养树突状细胞,并鉴定表面标志,分析DC表面标志与疾病活动指数之间的的相关性。方法采用密度梯度离心的方法分离外周血单核细胞,在培养瓶中贴璧3h,然后吸去悬浮细胞,联合应用GM-CSF,IL-4和TNF-α诱导分化,刺激正常人及SLE患者外周血DC增殖、分化、成熟。用免疫荧光标记培养的细胞,流式细胞仪分析DC各亚型,分析患者SLEDAI与DC各表型的相关性。结果SLE患者DC表达的CD1a,CD11c+,CD40和CD123百分率(58.88±7.64、54.4±10.88、37.29±8.08、13.14±4.44)较对照组(47.71±4.01、43.12±8.82、28.59±7.07、9.85±3.97)明显增加(P<0.05),SLE患者DC表达的CD80和CD83(55.16±10.12、57.76±11.54)较对照组(47.95±12.21、48.31±8.79)升高不明显(P>0.05)。SLEDAI与CD1a,CD11c+,CD40和CD123+呈明显相关性(P<0.05),与CD80和CD83相关性不明显。结论SLE患者DC表型表达增加,与疾病活动程度有密切关系。
Objective: To study the methods of efficient inducement and culture of peripheral blood dendritic cells (DC) in systemic lupus erythematosus (SLE) patients, and the identification of its surface marks and functions, to understand the relation of DC and the episode of SLE. Methods: The peripheral blood monocytes were separated by density gradient centrifugation, after 3 h of adhering, the suspend cells were transferred and induced with GM-CSF, IL-4 and TNF-α. The peripheral blood DC from normal control and SLE patients were stimulated to proliferate, differentiate and mature. The cultured cells were labeled with immunofluorescence, and the DC subsets were analyzed with flow cytometer, analyze the correlation between the DC subsets and disease activity. Results: The expressions of DC surface labels CD1a, CD11c+, CD40 and CD123 (58.88±7.64, 54.4±10.88, 37.29±8.08, 13.14±4.44) in SLE patients increased significantly than control groups (47.71±4.01, 43.12±8.82, 28.59±7.07, 9.85±3.97) (P<0.05), but the expressions of CD80 and CD83 (55.16±10.12, 57.76±11.54) in SLE patient groups and in control groups (47.95±12.21, 48.31±8.79) had no significant differences (P>0.05). SLEDAI were correlated significantly with CD1a, CD11c+, CD40 and CD123+ (P<0.05), but had no evident correlation with CD80 and CD83. Conclusion: The expressions of DC phenotypes were increased in SLE patients, and had affinity with disease activity.
出处
《中国医学工程》
2004年第5期18-20,23,共4页
China Medical Engineering
