摘要
借助离心式层折柱,成功地从琼脂糖凝胶中分离0.287～23.13kb范围的DNA片段;所得片段经乙醇沉淀、溶解后可有效地用于酶切、连接与转化。此法设备要求简单,操作省时简便,得率在50%以上。
With the spin chromatography column, the DNA fragments(0.287～23.13kb) were
separated successfully from agarose gel. After ethanol precipitation and resolution, the extracted
DNA fragments were good enough for digestion, ligation and transformation. This method is
simple, time-saving and the recovery rate is above 50%.
出处
《军事医学科学院院刊》
CSCD
北大核心
1993年第4期304-306,共3页
Bulletin of the Academy of Military Medical Sciences
基金
国家自然科学基金青年项目资助
关键词
DNA片段
分离
离心法
琼脂糖凝胶
separation of DNA fragment
centrifugation method
agarose gel
