摘要
目的 了解大鼠种植型肝癌电化学治疗 (EChT)后细胞免疫功能状态的改变。方法 应用流式细胞仪检测荷瘤大鼠治疗前后T细胞表面抗原 (CD3 、CD4、CD8)及应用免疫试剂盒检测外周血IL 2表达水平的变化 ,并与对照组进行比较。结果 荷瘤大鼠治疗前CD3 + 、CD4+ 、CD8+ 、CD4+ /CD8+ 、外周血IL 2表达水平均低于对照组。电化学治疗后CD3 + 、CD4+ 、CD8+ 、CD4+ /CD8+ 均有所回升 ,外周血IL 2水平回升。结论 荷瘤大鼠存在免疫功能低下 ,电化学治疗可纠正免疫紊乱 ,提高大鼠免疫功能。
Objective To study the changes of cellular immune function in rats with implanted liver tumor after electrochemotherapy (EChT). Methods The contents of T cell phenotype in blood was detected with flow cytometry (FCM) and serum interleukin-2 (IL-2) was measured using immune kits before and after EChT.The data were compared with those in the control group. Results Before EChT,CD_3 +,CD_4 +,CD_8 +,CD_4 +/CD_8 + and IL-2 in rats liver tumor model were lower than those in the control group.After EChT,CD_3 +,CD_4 +,CD_8 +,CD_4 +/CD_8 + in the rats model increased significantly ( P <0.05),and expression of serum IL-2 increased. Conclusion The cellular immunity of rats with implanted liver tumor was suppressed,and EChT could ameliorate cellular immune function of rats.
出处
《中国医学影像技术》
CSCD
2003年第5期517-518,共2页
Chinese Journal of Medical Imaging Technology
基金
国家自然科学基金资助课题 (30 0 70 2 33)
关键词
大鼠
肝癌
电化学治疗
免疫功能
Rat
Liver neoplasm
Electrochemotherapy
Immune function
