摘要
利用三抗体夹心ELISA(TAS-ELISA)及PCR检测的方法对采自海南的胜红蓟(Ageratum conyzoides)和假马鞭(Stachytarphetajamaicensis)的5个病样进行了检测,表明均为粉虱传双生病毒。PCR扩增产物克隆后进行序列测定,结果表明存在2类双生病毒,其中样品Hn2存在2类病毒的复合侵染。这是在海南首次报道存在有粉虱传双生病毒。
Five virus samples collected from Ageratum conyzoides and Stachytarpheta jamaicensis in Hainan were detected
with triple antibody sandwich ELISA (TAS-ELISA) and PCR, and the results showed that all the virus samples were infec-
ted by whitefly-transmitted geminiviruses. Sequence analyses showed two types of viruses existed among those samples, and
sample Hn2 was mix-infected by two types of viruses. This is Ihe first report of whitefly-transmitted geminiviruses in Hainan
Province.
出处
《植物病理学报》
CAS
CSCD
北大核心
2003年第6期513-516,共4页
Acta Phytopathologica Sinica
基金
国家杰出青年基金(30125032)