摘要
目的 调查中国浙江人群中FCGR3B的基因频率及基因变异。方法 聚合酶链反应 序列特异性引物方法 (PCR SSP)检测FCGR3B基因型。DNA测序分析FCGR3基因变异。结果 来自浙江的 4 87名健康中国人中 ,FCGR3B 1与FCGR3B 2的基因频率分别是 0 5 6 4及 0 4 2 9,而FCGR3B 3的基因频率为 0 0 0 0。FCGR3B基因缺失 (FCGR3Bnull)的频率为 0 6 2 % (3/487)。 19名中国人中的 7人存在FCGR3基因变异 ,表现为在第 3个外显子的多态性位点 14 1、14 7、2 2 7、2 6 6和2 77中发生 1个或多个单核苷酸改变。结论 在中国浙江人群中 ,FCGR3B 1基因频率高于FCGR3B 2 ,而FCGR3B 3可能缺失。单核苷酸改变所形成的新的基因变异在中国人群中也存在 。
Objective To investigate the FCGR3B gene frequencies and FCGR3 variants in a Chinese population from Zhejiang Province. Methods DNA was extracted from the blood specimens of 487 healthy blood donors from Zhejiang Province. The FCGR3B gene frequencies were determined by polymerase chain reaction with sequence-specific primers (PCR-SSP). The 19 specimens with 3 different FCGR3B genotypes underwent FCGR3 gene cloning and sequencing. Results The gene frequencies of FCGR3B*1, FCGR3B*2, and FCGR3B*3 in these 487 individuals were 0.564, 0.429, and 0.000 respectively. The genotype frequency of FCGR3B null was 0.62% (3/487). Sequencing of FCGR3 revealed that in 7 out of the 19 Chinese individuals variants caused by single nucleotide exchanges at one or more of the polymorphic positions 141, 147, 227, 266 and 277 in exon 3 also existed in this Chinese population. Conclusion FCGR3B*1 gene is more frequent in a Chinese population from Zhejiang Province than the FCGR3B*2 gene, and the FCGR3B*3 gene seems to be absent. Gene variants caused by single nucleotide exchanges are found in addition to the the well known forms, but the reason for this remains unclear.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2004年第17期1426-1428,共3页
National Medical Journal of China
基金
浙江省医药卫生科技计划基金资助项目 ( 2 0 0 4B0 45 )
浙江大学医学院科研启动基金资助项目 ( 4 910 10 5 42 92 2 )
