摘要
为探讨运动对心脏心钠素受体基因表达的影响 ,建立不同强度运动训练动物模型 ,采用免疫荧光组织化学法、原位杂交、激光共聚焦扫描和计算机图像分析技术 ,观察心脏心钠素受体的分布和运动对心脏心钠素受体基因表达的影响 ,结果表明 ,心钠素受体主要分布在心内膜、心肌纤维的肌膜、心肌纤维间的结缔组织和冠状动脉分支的血管壁。中等和大强度运动使心脏A型心钠素受体基因表达上调和C型心钠素受体基因表达下调 ,力竭运动使A型心钠素受体基因表达下调和C型心钠素受体基因表达上调。
To study the effect of exercise on gene expression of natriuretic peptide receptors (NPRs) in the heart, using immunofluorescent method, in situ hybridization, laser confocal scanning microscopy and the technique of computer image analysis, the distribution of NPRs in the heart and the alterations of gene expression of NPRs on the animal model of different training intensity were investigated. The result showed that NPRs located mainly in the endocardium, the sarcolemma of myocardial fiber, the connective tissue around myocardial fiber and the wall of coronary artery branch. After moderate and heavy exercise training, the gene expression of natriuretic peptide receptor-A (NPR-A) upregulated and natriuretic peptide clearance receptor (NPR-C) downregulated, whereas the gene expression of NPR-A downregulated and NPR-C upregulated after exhaustive exercise.
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2004年第4期386-389,412,i001,i002,共7页
Chinese Journal of Sports Medicine
基金
上海市教育委员会曙光计划项目 (项目编号 0 2SG3 6)
上海体育学院科学研究项目基金资助
关键词
运动
心脏
心钠素
受体
基因表达
ANP
心血管系统
exercise, natriuretic peptide receptors, gene expression, cardiac endocrine
