摘要
目的 观察脑室内注射一氧化氮合酶抑制剂 ( NOS) N-ω-硝基 - L -精氨酸 ( N- Arg)和一氧化氮 ( NO)供体药物硝普钠对大鼠学习记忆的影响。方法 应用 Y迷宫法观察 N- Arg和硝普钠对大鼠学习记忆能力的影响 ,应用分光光度法测定注射后大鼠大脑皮质、海马 NOS生物活性的变化。结果 与注射人工脑脊液的对照组相比 ,双侧脑室内注射 N- Arg后 ,除 5 mg组外 ,10 m g、15 mg和 2 0 mg组大鼠 Y迷宫学习能力均有不同程度下降 ,下降程度与脑室内注射 N - Arg的剂量相关。各 N- Arg注射组大鼠的记忆保持率与对照组比较没有显著差异。注射 N - Arg4 8h后可观察到海马 NOS生物活性降低。脑室内注射 N- Arg以后所引起的学习能力的降低可以被腹腔内注射1m g/kg的硝普钠所逆转。结论 中枢神经系统内 NO-
Objective To investigate the effects of intracerebroventricular infusion of nitric oxide synthase (NOS) inhibitor,N-ω-nitro-L-arginine (N-arginine,N-Arg),and NO donor,sodium nitroprusside (SNP), on learning and memory in rats.Methods The effects of N-Arg and SNP on learning and memory retention were evaluated by Y-maze.Changes of NOS biological activity in cortex and hippocampus were evaluated with spectrophotometric method.Results Compared with control rats, learning ability tested by Y-maze decreased in rats receiving 10 mg,15mg and 20mg N-Arg while N-Arg hadno effects on memory retention.NOS activity in cerebral cortex and hippocampus decreased 48h after intracerebroventricular infusion of N-Arg.The nitric oxide donor,SNP,alone had no effect on learning and memory.But intraperitoneal injection of SNP can attenuate the learning impairment induced by N-Arg.Conclusions The results of this study suggest that NO and NOS may play some roles in the learning and memory process of rats.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2004年第4期347-349,共3页
Journal of Apoplexy and Nervous Diseases
