摘要
目的 探讨妊娠高血压综合征 (妊高征 )患者血浆止凝血分子标志物水平变化的意义。方法 对 4 5例妊高征孕妇 (妊高征组 ,其中轻度 2 0例、中度 15例、重度 10例 )及 2 0例正常孕妇 (正常妊娠组 )分娩前后的血浆止凝血分子标志物进行检测。其中 ,采用酶联免疫吸附试验 (ELISA)检测两组孕妇分娩前后的P 选择素、凝血酶原片段 1+2 (F1+2 )、D 二聚体、纤溶酶抗纤溶酶复合物(PAP) ;采用发色底物法检测两组孕妇分娩前后的抗凝血酶活性。结果 (1)P 选择素 :妊高征组中、重度孕妇分娩前分别为 (6 6± 2 4 ) μg/L、(80± 30 ) μg/L ,正常妊娠组为 (49± 15 ) μg/L ,两组比较 ,差异有显著性 (P <0 0 5 )。分娩后妊高征组重度孕妇为 (6 5± 34) μg/L ,正常妊娠组为 (40± 12 ) μg/L ,两组比较 ,差异有显著性 (P <0 0 5 )。 (2 )F1+2 :妊高征组轻、中、重度孕妇分娩前分别为 (2 2± 0 2 )nmol/L、(2 3± 0 4 )nmol/L、(2 2± 0 2 )nmol/L ,均明显高于正常妊娠组的 (1 2± 0 3)nmol/L ,两组比较 ,差异有显著性 (P <0 0 5 )。 (3)D 二聚体 :妊高征组轻、中、重度孕妇分别为 (0 7± 0 1)mg/L、(0 7± 0 3)mg/L、(0 8± 0 2 )mg/L ,正常妊娠组为 (0 4± 0 1)mg/L ,妊高征组显著高于正常妊娠?
Objective To investigate the relationship between the hemostatic coagulation markers of pr ethrombosis state and pregnancy induced hypertension(PIH). Methods Forty-five PIH patients and 20 control patients were studied. P-selectin, pro thrombin fragments 1+2 (F1+2), D-dimers(D-D) and plasmin-antiplasmin complex (PAP) were measured by enzyme linked immunosorbent assay. Antithrombin activity (AT: A) was measured by chromogenic peptide substrate assay. Results (1) The P-selectin level of pre-delivery in moderate and severe PIH patients w as (66±24)μg/L and (80±30)μg/L, it was (49±15)μg/L in the control group ( both P<0.05). The P-selectin level of post partum in severe PIH group and control group was (65±34)μg/L and (40±12)μg/L, with significant difference between them( P<0.05). (2)The F1+2 level of pre-delivery in mild, moder ate and severe PIH groups was respectively (2.2±0.2), (2.3±0.4) and (2.2±0.2) nmol/L,being all significantly higher than that in the control gro up, which was (1.2±0.3) nmol/L, but there was no obvious difference between t hree PIH groups.(3)The D-D level in mild, moderate and severe PIH groups was r espectively (0.7±0.1), (0.7±0.3) and (0.8±0.2) mg/L, and it was (0.4 ±0.1) mg/L in the control group. The D-D level was increased when PIH became severe. (4)The PAP level in moderate and severe PIH groups was (0.8±0.4) mg /L and (0.8±0.4) mg/L, being significantly higher than that in control group (0.7±0.3) mg/L (both P<0.05). (5)The AT: Aactivity was obviously decre ased in PIH groups, being respectively (44±37) %, (64±25) % and (83±39) % in severe, moderate and mild PIH groups. There was obvious difference between seve re and mild groups (P<0.01). Conclusions Elevated P-selectin levels and increased pla telet activity were observed in PIH patients. F1+2 may be useful as a screening test for risk pregnancy. D-D can be used as an early monitor of DIC. AT: A r eflects the severity of illness. These molecular markers may be used to predict the prethrombosis state in PIH.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2004年第11期733-736,共4页
Chinese Journal of Obstetrics and Gynecology
基金
上海市科技发展基金资助项目 (9944 0 )
