摘要
目的 探讨表皮生长因子及其受体在中耳慢性鼓膜穿孔病变中的作用。方法在豚鼠慢性创伤性鼓膜穿孔的动物模型上 ,观察应用表皮生长因子治疗的鼓膜愈合率。在具有典型鼓膜后皱襞处穿孔的胆脂瘤型慢性化脓性中耳炎患者的病理标本上 ,应用免疫组化SP染色方法和计算机图像分析系统 ,检测 10例鼓膜穿孔部位邻近皮肤表皮生长因子受体的表达情况。结果 在动物实验中表皮生长因子治疗组鼓膜愈合率明显高于对照组 (χ2 =9 92 3,P <0 0 1)。未发现有诱发中耳胆脂瘤的病例。在临床研究中鼓膜穿孔邻近皮肤表皮生长因子受体的阳性率为 (39 3± 7 4 ) % ( x±s,下同 ) ,耳道深部正常皮肤为 (2 5 4± 3 7) %。 2组间差异具有高度显著性意义。结论 表皮生长因子有明显促进鼓膜穿孔愈合的能力。表皮生长因子受体在鼓膜穿孔部位邻近皮肤的中等表达 ,表明使用表皮生长因子治疗有一定的理论基础和有诱发中耳胆脂瘤的风险。
Objective To evaluate the possible roles of epidermal growth factor(EGF) and its receptor(EGFR) on the chronic tympanic membrane perforations. Methods A phosphate buffer saline of EGFR was administered to a Gelfoam pledget placed over chronic tympanic membrane perforations in guinea pigs. The EGFR of 10 specimens from the acquired middle ear cholesteatoma of the adjacent skin around perforation was examined by immunohistochemical SP method and computer image analysis. Results Complete closure of the tympanic membrane perforations was observed in 82.6% of EGF-treated ears, but only 33.3% in the controls(P<0.01).No case was led to middle ear cholesteatoma in the experiment group (0/23). The positive expression in the adjacent skin around perforation was (39.3±7.4)%; and the normal external ear skin was (25.4±3.7)%; There were distinctly significant differences between the adjacent skin around perforation and the normal external ear skin (P<0.01). Conclusions EGF is effective on closing chronic tympanic membrane perforations in the guinea pigs.Present data suggests that EGF-treated may induce the occurrence of middle ear cholesteatoma.
出处
《中华耳鼻咽喉科杂志》
CSCD
北大核心
2004年第11期669-671,共3页
Chinese Journal of Otorhinolaryngology
基金
温州市科委资助项目 (S2 0 0 0A3 2和Y2 0 0 3A10 3 )
关键词
表皮生长因子
鼓膜穿孔
慢性
治疗
受体
中耳
皮肤表皮
中等
结论
能力
Tympanic membrane perforation
Epidermal growth factor
Receptor,epidermal growth factor
Cholesteatoma,middle ear
