摘要
目的 利用基因芯片技术分析脂多糖 (LPS)活化的小鼠腹腔巨噬细胞基因表达谱 ,以更全面地了解LPS诱导的巨噬细胞反应。方法 以未刺激的和用 1mg/LLPS刺激的小鼠腹腔巨噬细胞制备3 3 P标记的cDNA探针 ,分别与含有 1176个已知基因的小鼠cDNA芯片杂交。结果 活化组和未刺激组间的 2倍差异表达基因为 118个 ,3倍差异表达基因为 6 9个 ,其中 4 4个上调 ,2 5个下调。转录因子、细胞内信号调节蛋白、炎症细胞因子和细胞凋亡相关基因的转录发生明显的调节变化。结论提供了LPS活化的巨噬细胞综合基因表达信息 ,并筛选出一些新的可能与LPS活化相关的基因。
Objective To make a more comprehensive knowledge of lipopolysaccharide (LPS)-induced inflammatory and immune reaction and the changes of gene expression profile of murine peritoneal macrophages after LPS activation were analyzed by cDNA microarry. Methods cDNAs were prepared from LPS-stimulated (1mg/L, for 2 h) and unstimulated murine peritoneal macrophages, radiolabeled with 33P, and hybridized with Atlas mouse cDNA microarry. Results Among the 1176 tested genes, 118 genes were found to be different by more than 2-fold between the groups, and 69 genes were different by more than 3-fold. Among them 44 genes were up-regulated and 25 genes were down-regulated. The expression of the genes encoding chemokines/cytokines was dramatically induced, such as IL-1β, MIP1, LIF and G-CSF. Many genes encoding transcription factor, cell signaling modulators and apoptosis associated proteins also changed their expression. In addition, the expression of those that were not previously linked to this murine model was also identified to be changed,such as BACH1, EGR2, EIP1, CNTF, RXRA, and adenosine A2 receptor. Conclusion This study has shown the systemic and comprehensive gene expression profile of LPS-activated murine peritoneal macrophages, and may contribute to better understanding of the mechanism of LPS or bacteria induced inflammatory and immune reaction. [
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2004年第10期773-777,共5页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金资助项目 ( 3 0 10 0 176)
