摘要
从家蚕品种菁松×皓月后部丝腺中提取总RNA ,用RT -PCR扩增技术克隆到家蚕丝心蛋白轻链cDNA .序列分析表明 ,该片段全长为 798个碱基 .比较 4种不同品种来源的家蚕丝心蛋白轻链基因外显子区域序列 ,同源性在98.0 %～ 99.4 %之间 ,推测的氨基酸同源性在 98.0 %～ 99.6 %之间 ,4个品种间发生的变异比较一致地集中在不同位置的 8个碱基上 .提取家蚕品种菁松×皓月后部丝腺总DNA ,进行PCR反应 ,获得家蚕丝心蛋白轻链基因调控片段 ,长度约为 1.2kb,测定了其邻近起始密码子一端包括 5 76个碱基的DNA序列 .结果表明 ,该片段包括一些典型的调控元件和多个可能参与丝蛋白基因特异性表达调控的元件 ,该部分序列与J - 139L链基因相应区域同源性高达 98.8% .图 3参
The fibroin light chain cDNA was cloned from the total RNA of Bombyx mori variety Jingsong Haoyue by RT-PCR. The cDNA contained 798 bp and encoded 262 amino acids. Sequences comparison of the corresponding exon region from 4 different Bombyx mori varieties showed that nucleic acid homology was between 98.0%～99.4%, the deduced amino acid homology was between 98.0%～99.6%, and the base variation was focused on 8 different sites. The promoter region (1.2 kb) of the fibroin light chain gene also was cloned by PCR, and partial sequence (576 bp) adjacent the initial codon was determined. The results indicated that it contained several typical elements of eukaryotic gene and several elements involved in expression regulation for fibroin gene, and the corresponding sequence of Jingsong ×Haoyue and J-139 showed 98.8% identity. Fig 3, Ref 8
出处
《应用与环境生物学报》
CAS
CSCD
2004年第5期614-617,共4页
Chinese Journal of Applied and Environmental Biology
基金
成都天友生物科技股份有限公司资助项目~~
