摘要
目的 体外培养人外周血单个核细胞 (PBMC) ,采用同型半胱氨酸 (HCY)和PPAR -γ激动剂(Troglitazone和Rosiglitazone)干预 ,探讨HCY和PPAR -γ与膜相关前列腺素E合成酶 (mPGEs)蛋白表达之间的关系。方法 抽取正常志愿者静脉血分离PBMC并培养 ,分别加入不同浓度的HCY及PPAR -γ激动剂培养 2 4小时 ,收集细胞培养液上清 ,采用ELISA测定mPGEs的水平。结果 HCY实验组mPGEs高于对照组 (P <0 .0 5 ) ,mPGEs水平随HCY剂量升高而升高 ,呈剂量依赖性 (P <0 .0 5 )。PPAR -γ激动剂对HCY10 0 μmol/L引起mPGEs水平的升高有明显抑制作用 (P <0 .0 1)。 结论 HCY促进PBMCmPGEs蛋白表达增加 ;PPAR -γ激动剂对HCY引起mPGEs水平升高有抑制作用。
Objectives To investigate the effect of homocysteine (HCY) on mPGEs. Expression of human peripheral blood mononuclear cell.Methods PBMC isolated from healthy volunteers, were cultured and intervened with HCY, Troglitazone and Rosiglitazone for 24 hours. mPGEs expression in the supernatants of cell culture were analyzed by ELISA. Results The OD value of mPGEs in HCY experimental groups were higher than in controls (P<0.05). The OD value of mPGEs increased with a HCY dosage-dependent style between 20μmol/L to 100μmol/L. All groups of Troglitazone and Rosiglitazone significantly reduced the level of mPGEs induced by 100μmol/L HCY (P<0.01), but there was no significant difference between the two PPAR-P activator groups (P>0.05). Conclusions HCY could stimulate PBMC to express mPGEs dosage-dependently. PPAR-γactivators could inhibit PBMC releasing and expressing mPGEs induced by HCY.
出处
《中国血液净化》
2004年第10期537-539,555,共4页
Chinese Journal of Blood Purification
基金
湖南省卫生厅重点项目资助 (Z0 2 0 6 )
