摘要
目的:探讨大鼠骨髓间充质干细胞(mesenchymalstemcells,MSCs)在诱导条件下的成骨特性。方法:使用密度梯度法分离成年大鼠骨髓MSCs,以地塞米松、β-甘油磷酸钠、维生素C为成骨诱导剂。结果:形态学表明,MSCs贴壁细胞呈集落生长,有成纤维细胞外观。地塞米松低剂量组诱导7d后碱性磷酸酶(alkalinephosphatase,ALP)表达明显升高,阳性细胞数为(15.1±2.6)个,而对照组ALP表达较弱,阳性细胞数为(12.0±3.5)个,两组比较P<0.01。地塞米松成骨诱导剂低剂量组促进MSC成骨,在诱导7d时即出现钙化结节,而对照组未出现;地塞米松低剂量组诱导21d钙化结节为(9.0±1.7)个,而对照组为(2.0±1.8)个,两组比较P<0.01。结论:低剂量地塞米松成骨诱导剂能诱导MSC向成骨细胞分化,可以为体内骨组织工程提供种子细胞。
Objective:To investigate the osteogenesis characteristics of cultured rat mesenchymal stem cells (MSCs) under bone induction condition. Methods: MSCs were isolated from adult rat by using density gradient separation method. The osteo-genic inducers were compounds of Dexone, β-glycerophosphate sodium and vitamin C. Results: The MSC attachment formed soon after the seeding and grew into colonies with the appearance of fibroblastic cells. The osteogenic inducer with low dose of Dexone could promote the osteogenic differentiation of MSC. In the group of osteogenic inducer with low dose of Dexone, the expression of alkaline phosphatase (ALP) was remarkably increased after one week's induction, and the number of positive cells was (15. 1 ± 2. 6) , significantly higher than that of the control group (12. 0 ± 3. 5) ( P<0. 01). The calcified deposits began to appear in the group of osteogenic inducer with low dose of Dexone after one week's induction and was increased remarkably after three weeks, and the number of calcified deposits was (9. 0 ± 1. 7) , significantly higher than that of the control group (2. 0 ± 1. 8) ( P <0. 01). Conclusion:MSC can differentiate into osteogenesis by osteogenic induction and may be used to provide seed cells for bone tissue engineering.
出处
《中西医结合学报》
CAS
2004年第5期375-378,共4页
Journal of Chinese Integrative Medicine
基金
国家自然科学基金资助项目(No.30271677
No.30371837)广东省自然科学基金资助项目(No.c031483)
