摘要
采用白菜子房培养和甘蓝胚培养方法得到了带有标记性状的白菜和甘蓝种间杂种,并将这些人工合成甘蓝型油菜回交于白菜,得到31个白菜-甘蓝单体附加系(2n=21),和18个双体附加系(2n=22),为进一步对附加染色体上的所载基因定位奠定了基础.此外,还研究了附加甘蓝染色体对雄性和雌性育性的影响,结果表明,白菜中附加甘蓝染色体可明显降低育性,特别是可显著降低附加系的自交和杂交结实率.
Artificially synthesized rapeseeds (Brassica napus) have been produced from the hybridization of B. campestris and B. oleracea by ovary culture and embryo culture. All accessions of B. campestris and B. oleracea have special morphological characters in order to locate those genes controlling the characters concerned. After backcrossing the synthetic B. napus to B. campestris, 31 plants with 2n = 21 and 18 plants with 2n = 22 chromosome number were developed. Further studies will be concentrated on the genes located on the added chromosomes of B. oleracea. Additionally, observation was made on the effects of the added chromosome(s) on the fertility of addition Lines. The result indicated that the added chromosome would apparently reduce the seed-setting percentage of addition lines.
出处
《华北农学报》
CSCD
北大核心
1993年第4期41-45,共5页
Acta Agriculturae Boreali-Sinica
基金
IFS国际科学基金资助项目
国家自然科学基金资助项目
关键词
油菜
染色体
育性
Synthetic rapeseed
Chromosome addition lines
Fertility
