摘要
目的:将体外培养的大鼠骨髓间质干细胞(mesenchymalstemcells,MSCs)移植到脑梗死大鼠梗死区,观察MSCs对脑缺血损伤的修复作用,探索一种治疗脑梗死的新方法。方法:建立Wistar大鼠大脑中动脉阻塞(MCAO)模型,将MSCs通过立体定向移植到脑梗死部位,并与假手术移植组、脑梗死对照组比较。结果:移植入脑的MSCs在正常脑组织与梗死区能存活至少4周以上,并发生迁移,至第3周移植治疗组迁移范围为(3.626±1.831)mm2,明显比假手术移植组(1.534±0.475)mm2大,差异有显著性意义(F=5.7863,P<0.05);接受MSCs移植后大鼠改良神经功能缺损量表(MNSS)得分低于另两组。结论:MSCs能在脑梗死区存活、迁移,并在一定程度上改善了脑梗死大鼠缺损的神经功能。
AIM:Mesenchymal stem cells(MSCs) cultivated in vitro were transplanted to infracted area of rats with cerebral infarction in order to observe the plerosis of cerebral ischemic impairment depended on NSCs and prove into a completely new method to treat cerebral infarction(CI). METHODS:Models of middle cerebral artery occlusion(MCAO) in Wistar rats were established.MSCs were transplanted to infracted area of brain stereotactically,for comparsion with sham operation transplantation group and CI control group. RESULTS:The transplanted MSCs survived for more than four weeks in normal and infarcted tissues of brain,and the transplantation occurred.Until the third week,the range of transplantation was(3.626±1.831)mm2 in transplantation group,which was obvious larger than that in sham operation group(1.534±0.475) mm2, and it was significant difference(F=5.786 3, P< 0.05).The scores of modified neurologic severity scores were lower after MSCs transplantation than those in other two groups. CONCLUSION:MSCs can survive and transplant in cerebral infracted area, and improve the neurologic functions in rats with CI to a certain extent.
出处
《中国临床康复》
CSCD
2004年第28期6060-6062,i002,共4页
Chinese Journal of Clinical Rehabilitation
基金
国家自然科学基金资助(C30100058)~~
