摘要
本文用普通ELISA和BA--ELISA检测牛肺疫血清抗体并与间接血凝试验和补体结合试验作比较,结果表明阳性检出率BA--ELISA(86.7%,13/15)>普通ELISA(80%,12/15)>间接血凝(53.3%,8/15)>补反试验(40%,6/15)。8种类症鉴别血清中6种呈现ELISA阴性。故认为ELISA是一种特异、敏感、快速、简便的血清学检测牛肺疫的方法,适于在现地检验时应用。
Serum Antibody of Bovine Contagious pleuropneumonia was detected by the application of ELISAand the results were compared with that of Indirect Hemagglutination and Complement Fixation Test. The results showed that the edtected rata was as follows,BA-ELISA(86.7%,13/15)>Conventional ELISA(80%, 12/15)>IHA(53.3%, 8/15)>CF(40%, 6 /15). Of8 differential diagnosis sera six sera were ELISA negtive.Therefore ELISA was considered to be one of the special,sensitive, fast and simple serological methods in detecting antibody and it can be used in diagnosis of quaratine.
关键词
牛
肺疫
血清抗体
检测
酶联免疫
Antibody of Bovine Contagious pleuropneumonia
Conventional ELISA
BA-LISA
Indirect Hemagglutination
