摘要
从山东、内蒙古、北京等地土壤中分离筛选出荧光菌 50 0 0余株 ,其中 1 2 0 0余株为抑制性荧光菌。经PCR检测 ,获得 73株 2 ,4 二乙酰基藤黄酚 (2 ,4 DAPG)产生菌。平板筛选结果表明 ,2 ,4 DAPG产生菌CPF 1 0和 2P8对沙打旺根腐病菌Sad1和Sad2均有较好的抑制效果 ,其中CPF 1 0的抑菌带宽分别为 5.0和 1 2 .0mm ;2P8抑菌带宽分别为 3 .5和 7.0mm。温室试验两次调查表明 ,CPF 1 0对沙打旺根腐病防治效果最好 ,达 63 .5%和 67.8% ;2P8防效也在 40 %左右 ,且均达到极显著水平。 2 ,4 DAPG产生菌可以显著促进沙打旺植株根系发育 ,CPF 1 0处理后地上部株高差异不显著 ,但鲜重和干重与对照相比有极显著的增加 ,说明两菌株菌剂处理可以促进植株生长。根部定殖结果表明 ,两菌株在沙打旺根部都有一定的定殖能力 ,在根表种群数量比较稳定 。
Totally ca. 5000 bacterial strains were isolated in soil samples from Shangdong, Inner-Mongolia and Beijing, in which ca. 1200 were identified as fluorecent antagonists against fungal pathogen Gaeumannomyces graminis which caused wheat take-all. By using PCR, 73 isolates were found to be able to biosynthesis antibiotic 2,4-diacetyphloroglucinol (2,4-DAPG), a key factor of biocontrol activities of some biocontrol Pseudomonas. Strain CPF-10 and 2P8 were employed in the further study due to their strong activity to inhibit the mycelial growth of milk vetch (Astragalus adsurgens Pall) wilt pathogen Fusarium spp. Sad1 and Sad2 on plate. Biocontrol test in greenhouse revealed that strain CPF-10 significantly suppressed the milk vetch wilt with the efficiencies of 63.5% and 67.8%, respectively, while the strain 2P8 showed ca. 40% biocontrol activity to above diseases. Compared with the control, the plants treated with CPF-10 or 2P8 showed an enhanced fresh weight and dry weight, indicating that these 2,4-DAPG producers promoted plant growth following the seed treatment. Colonization investigation also showed that both CPF-10 and 2P8 could stably colonize the rihizosphere of milk vetch and their endophytic populations gradually increased during the experimental period.
出处
《中国生物防治》
CSCD
北大核心
2004年第3期197-201,共5页
Chinese Journal of Biological Control
基金
国家 8 6 3项目 (2 0 0 3AA2 4 1170 )
关键词
沙打旺
根腐病
荧光假单胞杆菌
2
4-二乙酰基藤黄酚
定殖
Astragalus adsurgens
Fusarium spp.
Pseudomonas fluorescens
2,4-diacetylphloroglucinol
biocontrol
colonization
