摘要
测定了高密度R. eutropha细胞培养过程中二氧化碳的生成量。当通气量维持在1.0vvm时,出口气体中二氧化碳的最大浓度高达15%。为了了解高密度细胞培养过程中二氧化碳对细胞生长代谢可能产生的抑制作用,通过施加不同浓度、不同持续时间的二氧化碳脉冲,模拟测试了5升发酵罐内二氧化碳对R. eutropha细胞生长代谢的抑制作用。实验发现,二氧化碳对细胞生长的抑制作用随着二氧化碳脉冲时间的增长和浓度的增高而增强。当在细胞指数生长初期施加6小时浓度为22%的二氧化碳脉冲,最终细胞浓度降低了31%。
More CO2 would be evolved with the high cell density during fermentation processes. It is found that the dissolved carbon dioxide will affect the growth of many microorganisms. The evolution of carbon dioxide in fed-batch high cell density culture of Ralstonia eutropha was determined in a 5-L fermentor, and the maximum CO2 concentration of 15% in effluent gas was detected. CO2 pulsating additions with different pulse durations (3 or 6 hours) and concentrations (10%, 22%) were employed to study the CO2 inhibition on cell growth. It is found that the inhibitory effect of CO2 on cell growth is stronger with the increase of CO2 pulse duration and/or CO2 concentration. In the case of 6-hour 22% CO2 pulse, the final cell concentration (DCW) decreased 31% as compared to the results without CO2 addition. The amount of CO2 evolved mainly dependents on the cell culture concentration, so it is necessary to pay more attention on the large-scale high cell density culture for its occurrence of CO2 inhibition.
出处
《高校化学工程学报》
EI
CAS
CSCD
北大核心
2004年第5期633-637,共5页
Journal of Chemical Engineering of Chinese Universities
