摘要
目的 :制备抗HIVp2 4和人A型红细胞双特异性单抗 (mAb) ,并建立检测HIVp2 4的间接血凝试验。方法 :将分泌抗HIVp2 4mAb的杂交瘤株 2 E4和分泌抗人A型红细胞mAb的杂交瘤株S2 ,分别用 8 Ag和 5 BrdU驯化 ,使成为HAT敏感株。将两者常规融合 ,筛选分泌双特异性mAb的杂交 杂交瘤株。然后制备并纯化双特异性mAb ,用其建立的间接血凝法检测p2 4。结果 :共获得 6株杂交 杂交瘤细胞株 ,以其分泌的双特异性mAb建立了检测HIVp2 4的间接血凝法 ,敏感性可达 4 0 0ng/L。结论 :获得可稳定分泌双特异性mAb的杂交 杂交瘤株 ,并用纯化的双特异性mAb建立了快速检测HIVp2
AIM: To prepare bispecific monoclonal antibody (bsmAb) against HIV p24 and human group A erythrocytes, and set up an indirect hemagglutination test for detecting HIV p24. METHODS: Hybridoma cells 2-E 4 secreting anti-HIV p24 mAb and hybridoma cells S 2 secreting anti-human group A erythrocyte mAb were naturalized with 8-Ag and 5-BrdU respectively, making them sensitive to HAT. Then the two hybridoma cells sensitive to HAT were fused by routine method and hybrid-hybridoma cells secreting the bsmAb against HIV p24 and human group A erythrocytes were screened. The bsmAb was used to establish an indirect hemagglutination test for detecting HIV p24. RESULTS: 6 hybrid-hybridoma cell lines were obtained. An indirect hemagglutination test for detecting HIV p24 was set up by using the bsmAb, and its sensitivity reached 400 ng/L. CONCLUSION: The bsmAb against HIV p24 and human group A erythrocytes is prepared and a rapid indirect hemagglutination test for detecting HIV p24 is developed by using the purified bsmAb.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2004年第5期582-584,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
河北省科学技术研究与发展计划基金资助 (No.0 2 2 7640 1D)
