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KyoT2结合蛋白KBP1对RBP-J介导的转录活性的影响 被引量：1

Effect of KyoT2 binding protein KBP1 on RBP-J-mediated transcriptional activity
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摘要 目的 :研究KyoT2与KyoT2结合蛋白 1(KyoT2 bindingprotein1,KBP1)之间的物理相互作用 ,以及这种相互作用对重组信号结合蛋白J (recombinationsignalbindingprotein Jκ ,RBP J)介导的转录活性的影响。方法 :体外GST pulldown、体内免疫共沉淀、哺乳动物细胞双杂交实验和报告基因实验证实 ,KBP1与KyoT2之间存在物理和功能上的相互作用。结果 :体内和体外证实 ,KBP1与KyoT2之间均存在相互作用。转录活性分析实验显示 ,过表达KBP1可拮抗RING1对RBP J介导的转录的抑制效应。结论 AIM: To investigate the physical interaction between KyoT2 and KyoT2-binding protein 1 (KBP1) and the effect of KBP1 on RBP-J-mediated transcriptional activity. METHODS: GST-pull down, co-immunoprecipitation, mammalian cell two hybrid assay and reporter gene assay were used to examine the physical and functional interactions between KyoT2 and KBP1. RESULTS: KBP1 and KyoT2 could interact with each other both in vitro and in vivo, and overexpression of KBP1 could antagonize the suppressive effect of RING1 on RBP-J. CONCLUSION: KBP1 may indirectly modulate Notch signaling pathway by competing with RING1 for binding sites on KyoT2.
作者 秦鸿雁 韩骅
机构地区 第四军医大学基础部医学遗传学与发育生物学教研室
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2004年第5期544-547,共4页 Chinese Journal of Cellular and Molecular Immunology
基金 国家自然科学基金资助项目 (No .30 2 0 0 1 4 8 No .30 330 550 ) 第四军医大学博士学位论文课题资助 (No .2 0 0 2 0 0 5)
关键词 KyoT2 KyoT2结合蛋白1 重组信号结合蛋白J 转录 RING1 KyoT2 KBP1 RBP-J transcription RING1
分类号 Q756 [生物学—分子生物学]
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参考文献12

  • 1Li R, Wang JS, Sun Q, et al. Screening of molecules interacting with LIM domain protein KyoT[J]. Yi Chuan Xue Bao, 2002, 29(2): 175-180.
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同被引文献13

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  • 6Qin H, Wang J, Liang Y, et al. RING1 inhibits transactivation of RBP-J by Notch through interaction with LIM protein KyoT2[J].Nucleic Acids Res, 2004, 32(4): 1492-1501.
  • 7Whelan JT, Forbes SL, Bertrand FE. CBF-I (RBP-J kappa) binds to the PTEN promoter and regulates PTEN gene expression[J]. Cell Cycle, 2007, 6(1): 80-84.
  • 8Von Mutius E. The microbial environment and its influence on asthma prevention in early life[J]. J Allergy Clin lmmunol, 2016, 137(3): 680-689.
  • 9Ozdogan S, Tabakci B, Demirel AS, et al. The evaluation of risk factors for recurrent hospitalizations resulting from wheezing attacks in preschool children[J], ltal J Pediatr, 2015, 41: 91.
  • 10Prakash YS. Airway smooth muscle in airway reactivity and remodeling: what have we learned[J]. Am J Physiol Lung Cell Mol Physiol, 2013, 305(12): L912-L933.

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细胞与分子免疫学杂志
2004年 第5期

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