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RDP1258对大鼠脾细胞增殖及HO-1活性的抑制作用 被引量：2

Inhibitory effects of HLA-derived peptide RDP1258 on proliferation and heme oxygenase-1 activity of rat splenocytes
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摘要 目的 :观察一种新型HLA衍生肽 (RDP12 5 8)的免疫抑制作用 ,并探讨其机制。方法 :用化学方法合成一种HLA衍生肽(RDP12 5 8) ,以3 H TdR掺入法观察其在体外对大鼠脾细胞增殖的影响 ;以酶化学法观察其对脾细胞血红素氧合酶 1(hemeoxygenase 1,HO 1)活性的影响。结果 :RDP12 5 8可显著抑制大鼠脾细胞增殖并以剂量依赖的方式降低HO 1的活性。结论 :RDP12 5 8能够显著抑制丝裂原或同种抗原刺激所致大鼠脾细胞的增殖反应 ,其抑制作用可能是通过降低HO AIM: To observe the immunosuppression effect of a novel HLA-derived peptide RDP1258, and to explore its mechanisms. METHODS: RDP1258 was synthesized chemically. The effect of the peptide on alloreactive proliferation of rat splenocytes was observed by 3H-TdR incorporation method. The heme oxygenase-1(HO-1) activity was analyzed by the enzyme assay. RESULTS: The results showed that the synthetic HLA-derived peptide could obviously inhibit the proliferation of rat splenocytes and HO-1 activty in a dose-dependment manner in vitro. CONCLUSION: HO-1 may participate in the inhibitory effect of RDP1258 on the proliferation of rat splenocytes induced by mitogen or isoantigen.
作者 易善红 宋波 汪泽厚 张艮甫 崔志刚
机构地区 第三军医大学附属新桥医院泌尿外科
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2004年第5期632-634,共3页 Chinese Journal of Cellular and Molecular Immunology
基金 国家新药基金资助项目 (No.1 999A0 0 2 )
关键词 脾细胞 大鼠 增殖 HO-1 观察 HLA衍生肽 抑制作用 RDP 活性 化学方法 HLA-derived peptide mixed leukocyte culture heme oxygenase-1
分类号 R392 [医药卫生—免疫学] R541.4 [医药卫生—心血管疾病]
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参考文献8

  • 1Nisco S, Vriens P, Hoyt G, et al. Induction of allograft tolerance in rats by an HLA class-I-derived peptide and cyclosporine A[J]. J Immunol, 1994, 152(8):3786-3792.
  • 2Hanaway M J, Geissler EK, Wang J, et al. Immunosuppressive effects of an HLA class I-derived peptide in a rat cardiac allograft model[J].Transplantation, 1996, 61(8): 1222-1228.
  • 3Iyer S, Woo J, Cornejo MC, et al. Characterization and biological significance of immunosuppressive peptide D2702.75-84( E V) binding protein: Isolation of heme oxygenase-1 [ J]. J Biol Chem, 1998,273(5): 2692 -2697.
  • 4Paradela A, Alvarez I, Garcia-peydro PM, et al. Limited diversity of peptides related to an alloreactive T cell epitope in the HLA-B27-bound peptide repertoire results from restrictions at multiple steps along the processing-loading pathway[ J]. J Immunol
  • 5Magali G, Maria CC, Jean MN, et al. Decreased cytotoxic activity of natural killer cells in kidney allograft recipients treated with human HLA-derived peptide [ J ]. Transplantation, 1997, 63 ( 7 ): 1004 -1011.
  • 6Grassy G, Calas B, Yasri A, et al. Computer-assisted rational design of immunosuppressive compounds [ J ]. Nature Biot, 1998, 16 ( 8 ):748 - 752.
  • 7Woo J, Iyer S, Cornejo MC, et al. Stress protein-induced immunosuppression: Inhibition of cellular immune effector functions following overexpression of haem oxygenase ( HSP 32 ) [ J ]. Transplant Immunol, 1998, 6(2): 84 -93.
  • 8Ke B, Buelow R, Shen XD, et al. Heme oxygenase-1 gene transfer prevents CD95/Fas ligand-mediated apoptosis and improves liver allogrft survival via carbon monoxide signaling pathway [ J ]. Hum gen ther, 2002, 13(10): 1189-1199.

同被引文献17

  • 1赵宏伟,田秀珠,成要平,杨晓丽.Th1/Th2细胞因子在妊高征患者胎盘组织中的表达[J].现代妇产科进展,2004,13(5):368-370. 被引量：10
  • 2赵满仓,刘菊林,贾艳岩,卢新梅,段雪梅.载脂蛋白B单克隆抗体透射免疫比浊测定法的探讨[J].单克隆抗体通讯,1995,11(2):17-20. 被引量：1
  • 3Nisco S,Vriens P,Hoyt G,et al.Induction of allograft tolerance in rats by an HLA class-I-derived peptide and cyclosporine A[J].J Immunol,1994,152:3786-3792.
  • 4Hanaway MJ,Geissler EK,Wang J,et al.Immunosuppressive effects of an HLA class I-derived peptide in a rat cardiac allograft model[J].Transplantation,1996,61:1222-1228.
  • 5Iyer S,Woo J,Cornejo MC,et al.Characterization and biological significance of immunosuppressive peptide D2702.75-84(E V) binding protein:Isolation of heme oxygenase-1[J].J Biol Chem,1998,273:2692-2697.
  • 6Parham P,Clayberger C,Zorn SL,et al.Inhibition af alloreactive cytotoxic T lymphocytes by peptides from the α2 domain of HLA-A2[J].Nature,1987,325:625-628.
  • 7Magali G,Maria CC,Jean MN,et al.Decreased cytotoxic activity of natural killer cells in kidney allograft recipients treated with human HLA-derived peptide[J].Transplantation,1997,63(7):1004-1011.
  • 8Grassy G,Calas B,Yasri A,et al.Computer-assisted rational design of immunosuppressive compounds[J].Nature Biot,1998,16:748-752.
  • 9Redaelli CA,Wagner M,Kulli C,et al.Hyperthermia-induced HSP expression correlates with improved rat renal isograft viability and survival in kidneys harvested from non-heart-beating donors[J].transplantation,2001,14(6):351-360.
  • 10Ke B,Buelow R,Shen XD,et al.Heme oxygenase-1 gene transfer prevents CD95/Fas ligand-mediated apoptosis and improves liver allogrft survival via carbon monoxide signaling pathway[J].Hum gen ther,2002,13(10):1189-1199.

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细胞与分子免疫学杂志
2004年 第5期

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