摘要
目的 :研究乙肝Ⅱ号方对免疫性慢性肝损伤小鼠氧自由基 (OFR ) ,血栓素A2 (TXA2 )和前列环素(PGI2 )代谢的影响。方法 :将小鼠分为正常组、模型组和乙肝Ⅱ号方治疗组。对后两组小鼠用异种动物的肝提取物作为抗原 ,免疫纯系小鼠产生抗肝抗体 ,造成慢性实验性免疫性肝损伤模型。检测 3组小鼠肝组织丙二醛 (MDA)、超氧化物歧化酶 (SOD)含量及血浆TXA2 和PGI2 代谢产物的浓度。结果 :与正常组比较 ,免疫性慢性肝损伤小鼠肝组织MDA含量明显升高 ,且与血浆TXB2 变化呈正相关 (r =0 976,P <0 0 0 1)。肝组织SOD含量下降 ,血浆TXA2 明显升高 ,PGI2 明显下降 ;而乙肝Ⅱ号方治疗组小鼠肝组织MDA和血浆TXA2 较模型组低 ,SOD和PGI2 则较模型组高。结论
Objective:To study the effect of Prescription No Ⅱ to Anti-Hepatitis B on metabolism of oxygen free radicals (OFR) and TXA 2/PGI 2 in mice with Chronic Immuno-Hepatic Injury.Methods:The chronic hepatic injury was induced by injecting with antigen from liver extraction of heterogenous animal. The content of hepatic tissue malonyldialdehyde (MDA),superoxide dismutase (SOD) and the concentration of plasma TXB 2 and 6-keto-PGF1α were determined.Results:Compared with control animals, it was found that in hepatic tissue MDA level increased significantly and SOD content decreased markedly. The concentration of plasma TXB 2 increased significantly and 6-keto-PGF1α decreased markedly in mice with chronic hepatic injury. The level plasma TXB 2 was positively correlated with plasma MDA content( r =0.967, P <0.001).Conclusions:There was a synergic action between OFR and TXA 2-PGI 2 which might play an important role in the pathogenesis of chronic hepatic injury. Prescription No Ⅱ to anti-hepatitis B showed some inhibitory effect on changes of SOD, MDA, TXA 2 and PGI 2.
出处
《中西医结合肝病杂志》
CAS
2004年第5期278-280,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
基金
江苏省科委课题(No:BS96355)
关键词
小鼠
乙肝
PGI2
TXA2
慢性肝损伤
肝组织
免疫性
Hepatic Injury,Immunoreactivity
Prescription No Ⅱ
Malonyldialdehyde
Superoxide Dismutase
Prostaglandins
Mice, Inbred ICR
