摘要
目的 :观察柯萨奇 腺病毒受体 (CAR)在不同年龄、性别小鼠心肌组织中的表达情况。方法 :采用免疫组化和Western Blotting技术 ,半定量分析胚胎期 18d ,生后 1d ,4、8、12周雄性小鼠及 4周雌性小鼠心肌组织中的CAR含量。结果 :免疫组化半定量分析示胚胎期 18dCAR表达最高 ,生后 1d(新生鼠 )及 4周龄 (幼年鼠 )表达量依次降低 ,8周龄 (成年鼠 )基本不表达 (P <0 .0 5 ) ,12周龄 (老年鼠 )低表达 ,与 8周龄比较 ,差异有统计学意义 (P <0 .0 1) ,并且 ,4周龄雄性小鼠和雌性小鼠相比 ,CAR表达量雄性高于雌性 (P <0 .0 1) ,Western Blot ting结果示CAR表达变化趋势与免疫组化结果相一致。 结论 :小鼠心肌组织中CAR的表达具有明显的时序性 ,而且性别对CAR的表达有影响。
Objective: To investigate the expression of Coxsackie adenovirus receptor(CAR) in the myocardium of mice during various period of age and sex. Method:CAR expression levels 18 day in embryo, 1 day, 4 week, 8 week, 12 week after born in the myocardium of male mice and postnatal 4 week in the female mice myocardium were studied by Western-blotting and immunohistochemistry.Result: Immunohistochemistry semi-quantitative analysis showed CAR expression in mice myocardium was the highest on embryonic day 18, gradually decreased, absent at 8 th week after born and low at 12 th week. CAR expression in male mice myocardium at 4 th week was higher than that in female mice myocardium at 4 th week. Western-blotting showed that the changeable trend of CAR expression was the same as Immunohistochemistry.Conclusion:The expression levels of CAR in mice myocardium have obvious time course and sex can affect its expression.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2004年第10期600-602,共3页
Journal of Clinical Cardiology
基金
国家自然科学基金资助项目 (No :30 2 716 6 5 )
