摘要
用光学显微镜分析测试方法,对非晶颗粒态马铃薯淀粉不同条件下酶降解活性进行了观察和研究,结果发现,α 中温淀粉酶对非晶颗粒态淀粉生物活性作用能力高,使用较少量的酶作用于非晶颗粒淀粉,在颗粒表面上可以观察到明显变化。非晶颗粒态淀粉颗粒由于存在爆裂口,所以酶对非晶淀粉作用均从爆裂口开始,逐渐由爆裂口开始均匀扩张,颗粒模糊,最后颗粒消失。不同时间、酶量、温度对酶降解作用均产生一定影响。其中温度可明显加快酶降解速度,酶量的增加同样对酶降解产生显著影响。时间的延长也可以使非晶颗粒态淀粉逐渐降解,但变化较慢。
Using polarized light microscope analytical method,it was found that α-amylase at medium temperature has strong ability to degrade noncrystalline starch.Applying only a little enzymes to noncrystalline starch caused distinct change on the surface of granules.Beginning from the blowout poles of starch granules,starch granules were degraded deeply by α-amylase along the blowout poles to completion.Different duration time,amylase amount and temperature had some effects on α-amylase degradation activity.Degradation can be speeded by higher temperature,and increasing amount of α-amylase had the same effect.Prolonged time could make noncrystalline potato starch degrade,but the change was slow.
出处
《精细化工》
EI
CAS
CSCD
北大核心
2004年第10期763-767,共5页
Fine Chemicals
基金
国家自然科学基金资助项目(29976016)~~
关键词
马铃薯淀粉
非晶颗粒态
酶降解活性
potato starch
noncrystalline granular state
α-amylase degradation activity
