摘要
目的 观察不同浓度吗啡急性处理对脂多糖刺激后乳鼠离体星形胶质细胞活性的影响。方法 体外培养星形胶质细胞于融合状态 ,随机分为八组 :对照组 (L0 M0 组 )、0 5 μmol/L吗啡组 (L0 M0 5组 )、1 0 μmol/L吗啡组 (L0 M1 0 组 )、2 0 μmol/L吗啡组 (L0 M2 0 组 )、1 0 μg/ml脂多糖组(L1M0 组 )、1 0 μg/L脂多糖 +0 5 μmol/L吗啡组 (L1M0 5组 )、1 0 μg/ml脂多糖 +1 0 μmol/L吗啡组 (L1M1 0 组 )、1 0 μg/L脂多糖 +2 0 μmol/L吗啡组 (L1M2 0 )。在相应组中加入相应终浓度的吗啡和脂多糖 ,继续培养 2 4h。采用神经胶质纤维酸性蛋白 (GFAP)免疫组化方法分别检测星形胶质细胞免疫活性。结果 L0 M0 5、L0 M1 0 、L0 M2 0 组对正常星形胶质细胞形态均无影响 ;L1M0 组的星形胶质细胞GFAP免疫反应阳性细胞平均光密度 (AOD)明显高于L0 M0 组 (P <0 0 1) ;L1M1 0 和L1M2 0组GFAP阳性产物AOD值比L1M0 组均明显降低 (P <0 0 1)。
Objective To study the effect of acute morphine exposure on cultured astrocytes activity induced by lipopolysaccharide in rats.Methods Astrocytes were purified from 1 to 2 day old SD rats and divided into 8 groups.In group L 0M 0,L 0M 0.5,L 0M 1.0,and L 0M 2.0,0,0.5,1.0 or 2.0 μmol/L morphine was added to the cultured cells,respectively.In group L 1M 0,L 1M 0.5,L 1M 1.0,and L 1M 2.0,0,0.5,1.0 or 2.0 μmol/L morphine was added respectively to the cultured cells containing 1 μg/ml lipopolysaccharide.Then all cultured cells were incubated for 24 h.The immunoactivity of astrocytes was examined by means of immunohistochemistry methods.Results No significant difference of the immunoactivity of astrocytes was found between group L 0M 0 and the groups of L 0M 0.5,L 0M 1.0,L 0M 2.0.The glial fibrillary acidic protein(GFAP)positive cells of group L 1M 0 showed stronger staining than group L 0M 0,and the average of the optical density of GFAP immunoreactive cells(AOD) of group L 1M 0 was larger than that of group L 0M 0(P<0.01).Significant difference of AOD was found between group L 1M 0 and the groups of L 1M 1.0,L 1M 2.0(P<0.01).Conclusion The analgesic mechanism of rapid morphine treatment may be related to inhibition of the astrocytes activity.
出处
《临床麻醉学杂志》
CAS
CSCD
2004年第9期547-549,共3页
Journal of Clinical Anesthesiology
