摘要
目的研究胃癌组织中泛素(Ubiquitin)对细胞周期调控因子p27表达的影响及意义。方法采用组织芯片技术制作60例胃癌组织芯片,同时用S-P免疫组织化学和原位分子杂交(cDNA-mRNA)方法检测胃癌组织芯片中Ubiquitin、p27、p27mRNA的表达。结果60例胃癌组织中,Ubiquitin、p27、p27mRNA的阳性率分别为3.3%、40.0%、55.0%。淋巴结转移组的胃癌p27和p27mRNA阳性率显著低于无淋巴结转移胃癌(P<0.01)。Ubiquitin表达与p27低表达明显相关。结论应用组织芯片大规模高效检测临床组织样本是可行的,具有快速、方便、经济、准确的特点。p27的表达水平可以作为评估胃癌预后的参考指标,Ubiquitin影响p27的低表达。
Objective To study the effects of Ubiquitin on the expression of cell cycle regulatory factor p27 and analysis its meaning in gastric cancer. Methods By using tissue chip technique creating 60 cases gastric cancer tissue chip, at the same time using the S-P immunohistochemical and in situ hybridization methods, the expressions of Ubiquitin, p27 and p27mRNA in gastric cancer tissue chip was studied. Results The positive rates of Ubiquitin, p27 and p27mRNA were 43.3%, 40.0% and 55.0% in 60 cases gastric cancer respectively. The positive rates of p27 and p27mRNA of lymph node metastasis group were significantly lower than lymph node non-metastasis group (P<0.01). A significant correlation was found between the overexpression of Ubiquitin and low-expression of p27 in gastric cancer. Conclusion It is feasible to utilize tissue chip for a rapid, economic and accurate screening of clinical tissue specimens on a large scale. The expression of p27 might be considered to be a reference indicator for prognosis of gastric cancer. Ubiquitin has an ability to effect the low-expression of p27 in gastric cancer.
出处
《江西医药》
CAS
2004年第4期241-244,共4页
Jiangxi Medical Journal
基金
江西省卫生厅重大科技计划资助课题
