摘要
目的观察重组人胰高血糖素样肽 1[rhGLP 1(7 36 )NH2 和rhGLP 1(7 37) ]对实验性糖尿病大鼠模型血糖和血浆胰岛素水平的影响。方法正常SD大鼠按 3.2 4g/kg腹腔注射 5 0 %葡萄糖 ,诱发暂时性高血糖动物模型。腹腔注射不同剂量 (2 0、4 0、80 μg/kg)rhGLP 1(7 36 )NH2 和rhGLP 1(7 37) ,间隔取血测定血糖与血浆胰岛素水平。正常SD大鼠按 6 0mg/kg腹腔注射链脲佐菌素 ,建立实验性 1型糖尿病大鼠模型。静脉输注rhGLP 1(7 36 )NH2 [4.0ng/(kg·min) ]12 0min并观察血糖变化。结果腹腔注射 4 0、80 μg/kgrhGLP 1(7 36 )NH2 和rhGLP 1(7 37)处理组大鼠的血糖、血浆胰岛素水平与对照组之间的差别具有非常显著性意义 (P <0 .0 0 1) ,且呈现一定的量效关系。二者之间促胰岛素分泌和降血糖活性差异无显著性。静脉输注rhGLP 1(7 36 )NH2 6 0min后 ,血糖水平 (17.0 4± 1.31)mmol/L(P <0 .0 5 ) ,12 0min后血糖水平 (11.98± 1.0 5 )mmol/L(P <0 .0 0 1)。结论rhGLP 1能显著改善实验性糖尿病大鼠的血糖水平 ,这与其促胰岛素分泌活性有关。
PurposeTo study the effects of two recombinant human glucagon-like peptide-1 [rhGLP-1(7- 36)NH 2 and rhGLP-1(7-37)] on plasma glucose and insulin in the experimental diabetic SD rats.MethodsSD rat model with hyperglycemia was developed by intraperitoneal injection in these normal SD rats with 50%glucose 3.24 g/kg. rhGLP-1(7-36)NH 2 and rhGLP-1(7-37) were administered at different doses, then blood samples were collected and the levels of plasma glucose and insulin were determined. Subsequently, the experimental 1 model diabetic rats were produced with streptozotocin on 60 mg/kg. rhGLP-1(7-36)NH 2 [4.0 ng/(kg·min)] was intravenously injected into the diabetic rats for 120 min and the levels plasma glucose was measured.ResultsCompared with glucose group, plasma glucose levels were significantly decreased after administration 40,80 μg/kg rhGLP-1(7-36)NH 2 and rhGLP-1(7-37) in the experimental SD rat model. Besides, the levels of insulin in plasma were also apparently increased in these rats as compared with glucose rats (P<0.001). Both rhGLP-1(7-36)NH 2 and rhGLP-1(7-37) could improve the levels of plasma glucose and insulin, but these indexes showed no significant difference between them. In the type 1 diabetic rats, plasma glucose concentrations were(17.04±1.31) mmol/L (P<0.05) after 60 min by intravenous injection rhGLP-1(7-36)NH 2, and glucose concentrations were(11.98±1.05) mmol/L (P<0.001) after 120 min.ConclusionrhGLP-1 treatment could improve plasma glucose levels in the experimental diabetic rats model. It might be related to stimulating the secretion of plasma insulin in the rats model.
出处
《中国生化药物杂志》
CAS
CSCD
2004年第5期287-290,共4页
Chinese Journal of Biochemical Pharmaceutics
基金
山西省太原市科技局资助项目 (No .2 0 0 2 0 6)
