摘要
目的:分析本院2000年1月至2002年12月间产超广谱口内酰胺酶(ESBLs)大肠埃希菌耐药性的动态变化,为临床合理选用抗菌药物提供依据。方法:采用K—B纸片扩散法和ESBLs确证试验进行ESBLs的检测,数据输入WHONET5软件及SPSS 10.0软件进行统计分析。结果:2000年1月至2002年12月,本院所分离的389株大肠埃希菌中,产ESBLs的有163株,占41.9％;产ESBLs的大肠埃希菌对青霉素类、头孢菌素类、单环β内酰胺类、氨基糖苷类、喹诺酮类以及加β内酰胺酶抑制剂复合剂如:氨苄西林-舒巴坦、阿莫西林-克拉维酸、替卡西林-克拉维酸等抗茵药物呈多重耐药;显著高于非产ESBLs的大肠埃希菌(P<0.001);但对亚胺培南高度敏感。结论:本院产ESBLs的大肠埃希菌检出率高,临床治疗中应严格掌握抗茵药物应用指征,并动态监测其耐药性变迁,防止ESBLs大肠埃希菌的局部流行。
Objective : To investigate the trend of antibacterial resistance rate of extended-spectrum β-lactamases ( ESBLs ) producing Escherichia coli in our hospital from January 2000 to December 2002, for a guideline of rational use of antibacterial. Methods; Antimicrobial susceptibility were tested by Kirby-Bauer agar diffusion method and ESBLs were detected by disc diffusion confirmatory test. WHONET5 was used for data analysis and the SPSS 10.0 software was used for Chi-Square test. Results; 163 strains of E. coli (41. 9%) were considered ESBLs producing. The ESBLs-producing strains displayed multi-drug resistance to Penicillins, Cephalosporins, Monobactams, Aminoglycosides, Quinolones, Ampicillin-Sulbactam, Amoxicillin-clavulanic acid,Ticarcillin-clavulanic acid ect. . The resistance was significantly higher than that of non-ESBLs producing E. coli ( P < 0. 001). But both of them remained highly senstive to Imipenem. Conclusins ? The positive rates of ESBLs producing E. coli in our hospital was high and multi-drug resistance was serious. To prevent the ESBLs producing E. coli infection outbreak, more sputum culture and drug sensitivity should be emphasized before medication.
出处
《广州医学院学报》
2003年第4期19-22,共4页
Academic Journal of Guangzhou Medical College
基金
广州市科技局项目(No.2001-Z-064-01-2)
关键词
大肠埃希菌
产超广谱Β内酰胺酶
抗菌药物
耐药性
Escherichia coli
extended-spectrum
β-lactamases
antibacterial
drug resistance
