摘要
目的研究乙型肝炎病毒多表位治疗性疫苗基因的合成、表达及其产物的抗原性。方法设计、并合成乙型肝炎病毒多表位抗原基因BPT,克隆入融合表达载体pWR450-1,构建重组质粒PWR/BPT。在大肠杆菌中表达乙型肝炎多表位抗原蛋白并纯化该蛋白,并用Western-blotting方法初步检测该抗原蛋白的抗原性。结果成功构建了融合表达载体PWR/BPT,在大肠杆菌中表达出乙型肝炎多表位抗原蛋白,Western-blotting 检测显示该蛋白具有良好抗原性。结论HBV多表位治疗性抗原基因的设计是成功的,其在大肠杆菌中表达的重组融合蛋白具有良好的抗原性,可能是较理想的HBV治疗性疫苗候选物。
Objective To study the synthesis, cloning, expression and antigenicity of therapeutic multi-epitope gene of hepatitis B virus. Methods The therapeutic multi-epitope gene of hepatitis B virus was synthesized and cloned into the vector pWR450-1, then was expressed in E.coli and the products were purified. The immunogenicity of the expressed protein was analyzed by Western-blotting. Results Recombinant plasmid PWR/BPT was constructed successfully and the protein of multiepitope gene of hepatitis B virus was expressed in E.coli, which showed ideal antigenicity by Western-blotting. Conclusions The designed multi-epitope therapeutic gene of hepatitis B virus was proved to be correct and the expressed protein may be a good therapeu- tic vaccine.
出处
《第一军医大学学报》
CSCD
北大核心
2003年第8期802-805,共4页
Journal of First Military Medical University
基金
国家自然科学基金(30170532)
广东省自然科学基金(990375)
广州市重点攻关课题(2001-2-035-01)~~
关键词
乙型肝炎
多表位
治疗性疫苗
hepatitis B virus, multi-epitope, therapeutic vaccine
