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极端嗜热菌Thermotoga hypogea细胞抽提液中醇脱氢酶和氢化酶的活性检测 被引量：1

The Alcohol Dehydrogenase and Hydrogenase from Cell-free Extract of Thermotoga hypogea,A Hyperthermophilic Bacterium
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摘要 　极端嗜热菌Thermotogahypogea(T.h.)是一株来自中非油田井下的,能够生长在90℃高温下的短杆状厌氧嗜热菌,经研究发现,在T.h.菌体细胞抽提液中存在一种特殊的NADP依赖性醇脱氢酶(ADH)。该酶在T.h.菌的醇醛代谢途径中起着关键性的催化作用,可催化多种醇醛底物的脱氢反应。同时,当以二氯联卞吡啶为作用底物时,T.h.菌的细胞抽提物显示很高的氢化活性(Km=0.29mmol·L-1,Vmax=41.69)。 Thermotoga hypogea (T.h.), which came from an midAfrica oilproducing well,is a Hyperthermophilic bacterium that is able to grow at 90 ℃. A NADPdependent alcohol dehydrogenase (ADH) is found to be present in the cytoplasmic fraction of the cell of T.h. in this research. The results indicate that this ADH can be a key enzyme in the metabolic pathway of alcohol production. The cellfree extract of T.h. also shows strong hydrogenase activity with Benzyl viologen dichloride as the substrate(Km=0.29 mmol·L-1, Vmax=41.67)
作者 王颖 吕巡贤 K.Ma
机构地区 新疆农业大学药学学院 新疆农业大学环境资源学院 加拿大滑铁卢大学生物系
出处 《新疆农业大学学报》 CAS 2003年第3期75-78,共4页 Journal of Xinjiang Agricultural University
关键词 极端嗜热菌 THERMOTOGA hypogea 醇脱氢酶 氢化酶 hyperthermophilic bacterium Thermotoga hypogea alcohol dehydrogenase hydrogenase
分类号 Q93335 [生物学—微生物学]
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参考文献5

  • 1王颖,L.Nie,K.Ma.极端嗜热菌Thermotoga hypogea生长条件的研究[J].新疆农业大学学报,2003,26(1):51-54. 被引量：1
  • 2Winker S ,C R Woese.A Definition of the domains Archaea, Bacteria and Eucarya in terms of small subunit ribosomall RNA characteristics[J].Syst. Appl. Microbiol.,1991,13: 161～165.
  • 3M L Fardeau,B Olliver.Thermotoga hypogea sp. Nov.,a Xylanolytic, Thermophilic Bacterium From an Oil-producing well[J].International Journal of Systematic Bacteriology,1997,47:1013～1019.
  • 4Ravot G,B. Olliver.Thiosulfate Reduction ,an Important Physiological Feature Shared by Members of the Order Thermologales[J].Applied and Environment Microbiology,1995,61:2053～2055.
  • 5Ma Kesen,Frank T Robb.Purification and Characterization of NADP-Specific Alcohol Dehydrogenase and Glutamate Dehydrogenase from the Hyper thermophilic Archaeon Thermococcus Litoralis[J].Applied and Environment Microbiology, Feb.1994,60:562～568.

二级参考文献5

  • 1Winker S,C R Woese. Syst. Appl. Microbiol. 1991,13 :161--165.
  • 2Fardeau M L,B Olliver. International Journal of Systematic Bacteriology[J]. 1997,47: 1013-1019.
  • 3Ravot G,B Olliver. Applied and Environment Microbiology[J], 1995,61:2053- 2055.
  • 4Ma K,Frank T. Robb. Applied and Environment Microbiology[J], 1994,60 : 562 - 568.
  • 5Reid M F,C A Fewson. Crit. Rev. Microbiol., 1994, 20 : 13.

同被引文献15

  • 1李秋波,邢德峰,任南琪,赵丽华,宋业颖.C/N比对嗜酸细菌X-29产氢能力及其酶活性的影响[J].环境科学,2006,27(4):810-814. 被引量：23
  • 2Das D., Veziroglu T.N. Hydrogen Production by Biological Processes: a Survey of Literature [ J ]. Int. J. Hydrogen Energy, 2001, 26:13-28.
  • 3Brosseau J.D., Xajic J. E. Continuous Microbial Production of Hydrogen Gas [J]. Int. J. Hydrogen Energy. 1998,23: 559-563.
  • 4Y. Ueno, S. Otsuka, M. Morimoto. Hydrogen Production from Industrial wastewater by Anaerobic Microflora in Chemostat Culture [J]. J. Ferment. Bioeng. 1996,82 ( 2 ) : 194 - 197.
  • 5S. Tanisho, Y. Ishiwata. Continuous Hydrogen Production from Molasses by the Bacterium Enterobacter agrogenes [J]. Int. J. Hydrogen Energy. 1994,19(10) : 807-812.
  • 6Nicolet Y., Lemon B.J., Fontecilla - Camps J.C., Peters J.W. A Novel FeS Cluster in Fe- Only Hydrogenases [J]. Trends Biochem. Sci.2000,25:138-143.
  • 7P. Tamagninl R., Axelsson P., Lindberg F., Oxelfelt R., Wtlnschiers P., Lindblad. Hydrogenases and Hydrogen Metabolism of Cyanobacteria [J].Microbiol. Mol. Boil.Rev.2002,66:1-20.
  • 8Fernando A,Lopes P, Olga T,et al. A brief look at three decades of research on cyanobacterial [J]. Int.J.Hydrogen Energy,2002,29:1209- 1215.
  • 9N. Q. Ren, B. Z. Wang, F. Ma. Hydrogen Bio-production of Carbohydrate Femrentation by Anaerobic Sludge Process. Proceedingsof 68th Annual Water Environmental Federal Conference. Khrwer Academic/Plenum Publisher, 1995, Miami : 145-152.
  • 10Defeng Xing, Nanqi Ren, Xie Tianhui, et al. Continuous hydrogen produetion of auto - aggregative Ethanoligenens harbinense YUAN - 3 in a continuous stirred - tank reactor. 16th World Hydrogen Energy Conferenee. Lyon, 2006 (Oral presentation).

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新疆农业大学学报
2003年 第3期

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