摘要
目的 探讨雌激素受体 (ER)、孕激素受体 (PR)、血管内皮生长因子 (VEGF)及其受体 (KDR)在宫内节育器 (IUD)致子宫异常出血中的作用。 方法 对放置IUD后异常出血的子宫内膜 (IUD出血组 )和正常子宫内膜(正常对照组 )进行免疫组织化学染色 ,检测内膜组织中ER、PR、VEGF、KDR的含量 ,并以抗第八因子相关抗原 (F8 AR)抗体标记间质微血管 ,进行微血管计数。 结果 1 IUD出血组ER表达明显强于正常对照组 (P <0 0 5 ) ;PR的表达则无显著性差异 (P >0 0 5 )。 2 IUD出血组VEGF和KDR的表达明显强于正常对照组 (P <0 0 0 1)。 3 IUD出血组微血管密度 (MVD)明显高于正常对照组 (P <0 0 0 1)。 结论 IUD所致的子宫异常出血与ER、VEGF和KDR表达的增多所导致的MVD的增高有关。
Objective To investigate the effects of oestrogen-receptor(ER), progesterone-receptor(PR), vascular endothelial growth factor(VEGF) and kinase insert domaincontaining receptor(KDR) in uterine bleeding induced by intrauterine device(IUD). Methods Immunohistochemical method was used to detect the expressions of ER, PR, VEGF and KDR in endometrium from IUD bleeding group and normal control group, respectively. Microvessel density(MVD) was evaluated by using anti factor Ⅷ-related antigen (F8-RA) antibody to mark stromal microvessels. Results The expressions of ER, VEGF and KDR were significantly higher in IUD-induced endometrium bleeding group than those in control group(P<0.05). The expression of PR was no significant difference in endometrium between two groups(P>0.05). MVD was significantly higher in IUD-induced endometrium bleeding than that in normal endometrium(P<0.001).Conclusion The increases of ER, VEGF and KDR contents result in the increase of MVD, which may be related to abnormal bleeding in IUD users.
出处
《解剖学报》
CAS
CSCD
北大核心
2004年第4期430-433,共4页
Acta Anatomica Sinica
基金
北京市卫生局基础 临床结合基金资助 ( 2 0 0 1 0 3 )
