摘要
目的 分析 134株超广谱 β -内酰胺酶菌株的耐药性 ,以利于药物治疗。 方法 应用双纸片协同试验鉴定超广谱 β-内酰胺酶菌株 ,琼脂稀释法检测超广谱 β-内酰胺酶细菌的最低抑菌浓度 (MIC)。 结果 亚胺培南敏感率 85 8% ,10 7株菌的MIC在 0 12 5～ 4 0 μg/ml之间 ,美洛培南敏感率 94 0 % ,12 6株菌的MIC在 0 12 5～ 4 0 μg/ml之间 ;头孢哌酮 /舒巴坦敏感率 4 0 3% ,4 4株菌的MIC在 0 12 5～ 16 0 μg/ml之间 ;哌拉西林 /他唑巴坦敏感率5 6 0 % ,5 9株菌的MIC在 0 12 5～ 16 0 μg/ml之间 ;头抱西丁敏感率 4 7 0 % ,6 3株菌的MIC在 0 12 5～ 8 0 μg/ml之间 ;左氧氟沙星敏感率 6 1 9% ,83株菌的MIC在 0 12 5～ 2 0 μg/ml之间 ;环丙沙星敏感率 5 3 7% ,72株菌的MIC在0 12 5～ 1 0 μg/ml之间 ;丁胺卡那霉素敏感率 6 4 2 % ,86株菌的MIC在 0 12 5～ 8 0 μg/ml之间 ;氯霉素、头孢噻肟、头孢吡肟、氨曲南敏感率均在 4 0 0 %以下。结论 抗菌活性最好的是碳青霉烯类药物 ,加酶抑制剂的头孢哌酮 /舒巴坦、哌拉西林 /他唑巴坦中介耐药增加 ,主要原因是临床过度使用所致 ,喹诺酮类抗生素对大肠埃希菌以外的细菌抗菌活性较好。
Objective To analyse the resistance of strains producing ESBLs so as to give a better medicine therapy.Methods Strains produing ESBLs were identified by double-disk synergy test and their MIC were detected by agar dilusion test.Results The sensitivity of Imipenem was 85.8% and MIC was between 0.125-4.0μg/ml;Meropenem 94.0% and 0.125-4.0μg/ml .They had shown good antibiotic activity.Cefoperazone/Sulbactam 40.3%,MIC at 0.125-16.0μg/ml (44 strains);Piperacillin/tazobactam 56.0%,MIC 0.125-16.0μg/m(59 strains);Cefoxitin 47.0%,MIC 0.125-8.0 μg/ml(63 strains);Levofloxacin 61.9%,MIC 0.125-2.0μg/ml(83 strains);Ciproaoxacin 53.7%,MIC 0.125-1.0 μg/ml(72 strains);Amikacin 64.2%,MIC 0.125-8.0 μg/ml(86 strains);Chloramphenicol,Cefotaxime,Cefepime and Aztreonam all below 40.0%.Conclusions Carbapenems had the best antibiotic activity.There were increases of intermediate resistane for Cefoperazone/sulbaetam and Piperacillin/tazobactam which have enzyme inhibitor.They have been overused in clinical may be the main reason.Quinolones have better antibiotic activity on bacteria except for Escherichia coli.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2004年第4期484-485,共2页
Chinese Journal of Public Health
