摘要
目的:观察长期雌激素缺乏去卵巢大鼠海马结构组织形态及神经元数量的变化,同时观察比较长期口服小剂量17-β雌二醇和复方尼尔雌醇对去卵巢大鼠海马结构组织形态及神经元数量的作用及效果。方法:7月龄SD大鼠随机分成5组:正常对照组(正常组)、假手术组(SHAM组)、去卵巢组(OVX组)、17β-雌二醇预防干预组(OVX/ERT组)和复方尼尔雌醇预防干预组(OVX/NL组)连续观察。5组均在去卵巢后35周处死。大体观察大脑及海马结构的形态,秤量大脑质量。常规苏木精-伊红染色和Nissl染色,光镜下观察海马结构的组织形态及计数各亚区神经细胞数。结果:各组大鼠大脑表面的脑沟回形态正常,冠状切面上弓形隆起的海马大小形态正常,未见明显的萎缩变小。各组间大脑质量差异无显著性意义。苏木精-伊红染色和Nissl染色示各组间神经元在细胞形态、大小和排列上未见明显差别。海马结构各亚区神经元数量、截面积和周长在各组间差异无显著性意义。结论:去卵巢衰老大鼠缺乏阿尔茨海默病所具有的明显的脑萎缩、脑质量的下降以及海马结构内神经元数量的减少,未发现去卵巢大鼠行为学异常的组织形态学基础。
AIM:To observe the changes in the histological features of hippocampal structure and the number of neurons in ovarietomized rats with long-term estrogen deficiency,and to observe the effect of long-term oral medication of low-dose 17 beta(β)-estradiol and compound nilestriol tablet on the histological features of hippocampal structure and the number of neurons. METHODS:Fifty 7-month-old female Sprague-Dawley rats were divided into five groups randomly: normal control group(normal group),sham operated group(sham group),ovarietomized(OVX)group,OVX plus 17β-estradiol(OVX/ERT)group and OVX plus compound nylestriol tablet(OVX/NL) group.All the rats were killed 35 weeks after being ovarietomized, the brain and hippocampal formation were observed,and then the cerebrum mass was measured.HE staining and Nissl staining were performed,the histological features of hippocampal formation were observed under light microscopy,and the number of nerve cells in all subregions was counted. RESULTS:Normal form of sulci and gyri was observed on the surface of cerebrum,and the size and form of hippocampus with arcuate eminence on the coronal cut were also normal,and there was no obvious atrophy in rats of all groups.There was no difference in cerebrum mass among all groups.The results of HE and Nissl stainings showed that there was no obvious difference in the cell form, size and arrangement of neurons among all group. The number,cross-sectional area and perimeters of neurons in all subregions of hippocampal formation were not significantly different among all groups. CONCLUSION:No significant gross morphological changes of Alzheimer disease such as brain atrophy,decreases of cerebrum mass and neuron loss are observed in ovarietomized rats.
出处
《中国临床康复》
CSCD
2004年第25期5259-5261,i001,共4页
Chinese Journal of Clinical Rehabilitation
基金
"十五"国家医学科技攻关课题(2001BA702B03)~~
