摘要
目的 研究人的年轻恒牙和成熟恒牙牙髓中碱性成纤维细胞生长因子 (bFGF)的表达及分布特征 ,探讨bFGF在恒牙牙根发育过程中的作用。方法 对牙髓标本的石蜡切片进行bFGF的免疫组化染色 ,并做图像定量分析。结果 bFGF在牙根刚开始发育、发育过程中及发育完成后的牙髓中的阳性表达呈逐渐减弱。结论 bFGF在人正常发育的牙髓组织中 ,随着牙根的逐渐发育而呈现不同特征的表达 ,因而参与牙髓的发育和成熟。
Objective The purpose of this study was to investigate the changes of basic fibroblast growth factor (bFGF) expressed in normal human dental pulp at different root development stages of permanent teeth.Methods Based on the teeth root development status, the pulp tissues were classified into three groups: root just starting development, being in development and apical closed. The pulps were immunohistochemically examined by use of bFGF antibody.Results Staining was strongly positive in immature permanent teeth, especially at the stage of root just starting development. Image analysis indicated that the gray values of positive reaction in three groups were statistically different (P<0.001). For the first group, the gray value of the outer pulp was higher than that of the pulp core. For the second group, the pulp core has a higher gray value in the coronal pulp, while a lower value in root pulp compared to the outer pulp.Conclusion With the development of root formation, the expression of bFGF in dental pulp shows different characteristics. bFGF may play a role in dental pulp development and maturation.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2004年第4期271-274,共4页
West China Journal of Stomatology
