摘要
本文用7株单克隆抗体(McAb),疟疾流行区感染病人免疫血清和BALB/c小鼠免疫血清对用^(35)S—蛋氨酸和^(125)Ⅰ—表面标记的恶性疟原虫无性红内期保护性抗原进行了分析。实验结果表明,抑制性McAbs和不同来源的多价免疫血清巳鉴定出10余种功能性靶抗原。两种不同来源的多价免疫血清不但能识别由这7株McAbs所识别的靶抗原而且还能识别140和100KDa以及某些较小分子量的多肽。同时还用抗原竞争试验证实了免疫沉淀靶抗原的特异性。
Seven monoclonal antibodies (McAbs) Specific for Plasmodium falciparum, human immune sera of three individuasl,two adults and one child, living in a malaria endemic area in Hainan Island of China and immune sera of BALB/c mice mimunized with the erythr-
ocytic stages of Platmodium falciparum were used to identiffy the protective antigens of the asexual blood stages of Plasmodium falciparum. The human immune sera from the adults living in a malaria endemic area and mouse immune sera not only immunprecipitated the target antigens which were recognized with the McAbs but also the polypeptides of 140,100 and some lover-molecular weights. At the same time, the specificity of imm-
unoprecipitated antigens was confirmed with the antigen competition assay.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1989年第2期33-36,共4页
Immunological Journal
关键词
恶性疟原虫
单克隆抗体
疟原虫抗原
Pasmodsum falciparum,Monoclonal antibody,Malaria antigens
