摘要
本文系用人肺腺癌细胞株A549为研究对象,观察了TDSF对T淋巴细胞的作用,并对其某些生物学特性作了初步研究。实验表明:TDSF对T淋巴细胞的增殖、IL-2的产生及其反应性均有明显的抑制作用。TDSF作用相当强烈,这点是应用IL-2对肿瘤进行免疫治疗时应该注意的问题。丝裂霉素等抗代谢药物可抑制TDSF的分泌,提示TDSF为瘤细胞合成和分泌的基因产物。TDSF对酸、碱、热、胰蛋白酶敏感,分子量>150KD,表明其化学本质为蛋白质。
Department of Medical Mclcuiar Biology,Tongji Medical University,Wuhan
Using lung cancer cell line,A549,the effects of tumor-derived immunosupp-ressive factor (s) (TDSF) on T lymphocyte and its biologic characters have been investigated. The results showed TDSF was able to inhibit PHA-stimulated proliferation of T lymphocytes,block the production of IL-2 from PHA-stimulated lymphocytes and inhibit IL-2 dependent proliferation of activated lymphocytes. The fact that TDSF possess very strong inhibitive action on IL-2 is especially noticeable if we consider using IL-2 as immunotherapeutic agent. The synthesis of TDSF from A549 cells was inhibited by mitomycin C and other metabolic inhibitors. These results indicate that TDSF is genie products of A549 cells. TDSF is protein in chemical nature with a molecular weight greater than 150Kd and sensitive to pH,heat and trypsin treatment.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1989年第1期10-14,共5页
Immunological Journal
基金
中国科学院自然科学基金
关键词
免疫
抑制因子
癌细胞株
IL-2
A549 culture supernatant,Tumor-derived immunosuppressive factor,Interieukin 2
