摘要
目的 研究雌激素 β受体 (estrogenreceptorβ ,ER β)在妊娠及哺乳期小鼠下丘脑室旁核 (paraventricularnucle us ,PVN)内表达的变化情况。方法 硫酸镍铵增强显色的免疫组化 (S P)法观察前述变化情况。结果 ER β免疫阳性物质主要见于PVN的大细胞部 ,在小细胞部和帽部免疫阳性细胞数目较少。免疫阳性物质主要位于整个细胞核内 ,未见明显的胞浆或突起免疫阳性反应。在妊娠中期ER β的表达最低 ,随后升高 ;最高表达见于妊娠第 18天至分娩后 1d ,随后表达降低到成年水平。结论 在妊娠和哺乳过程中PVN内最高水平的ER β表达见于分娩前后很短的时间内 ,提示PVN内ER
Objective To study the expression of estrogen receptor beta (ER β) in the hypothalamic paraventricular nucleus (PVN) of maternal mice during pregnancy and lactation. Methods Nickel ammonium sulfate intensified immunocytochemical method was employed to investigate the expression of ER β in the postnatal developing PVN of female mice. Results ER β immunopositive materials were predominantly localized in the magnocellular division of PVN, and sparse positive cells were found in the parvocellular division. Most of the positive materials were found in the whole cell nuclei, but no obvious cytoplasma or process immunopositive cells were detected. In the pregnant female brain, generally, the ER β level was lower. The lowest levels of expression were found at mid pregnancy, and then peaked at peripartum (from gestational days 18 to postpartum day 1), followed by another decrease to normal adult level from postpartum day 4. Conclusion The above results suggest that during pregnancy and lactation, ER β may be predominantly involved in the PVN regulation of parturitional process.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2004年第15期1339-1341,共3页
Journal of Third Military Medical University
基金
第三军医大学中青年科研基金资助项目 ( 2 0 0 2 )~~
关键词
雌激素
雌激素受体
室旁核
妊娠
哺乳
estrogen receptor
immunocytochemistry
paraventricular nucleus
pregnancy
lactation
