摘要
采用双波长薄层扫描法对祛痹舒肩丸中的有效成分延胡索乙素的含量进行测定。展开剂 :正己烷-醋酸乙酯 -浓氨试液 (3∶2∶0 1) ;扫描波长 :测定波长λs =5 15nm ,参比波长λR=6 5 0nm ;狭缝 :0 4mm× 0 4mm ;线性参数 :SX =3。延胡索乙素线性范围 0 4 8～ 3 84 μg ,r =0 9994 ,平均回收率为 10 0 98% ,RSD为 1 77% (n =8)。本法重复性好 ,定量准确 ,可作为该制剂的定量分析方法。
A TLC-scanning method for the determination of dl-Tetrahydropalmatine in Qubi Shujian pellets (a Chinese Traditional Medicine for curing paralysis and comfortizing shoulders) was established. The developing agents were n-hexane-acetic ether- strong ammonia solution (3∶2∶0 1).The testing wavelength λs=515nm, reference wavelength λ R=650nm, the slip was 0 4mm×0 4m and linear parameter was SX=3. The calibration curve was linear in the range of 0 48～3 84 μg (r=0 9994) and average recovery rate was 100 98%, RSD=1 77% (n=8). The method was accurate, repeatable, and could be used as a quantitative method for the determination of dl-Tetrahydropalmatine in Qubi Shujian pellets.
出处
《中国药事》
CAS
2004年第8期495-497,共3页
Chinese Pharmaceutical Affairs
关键词
祛痹舒肩丸
延胡索
延胡索乙素
含量测定
薄层扫描
Qubi Shujian pellets
dl-Tetrahydropalmatine
content determination
TLC-scanning
