摘要
目的 :研究不同浓度丙泊酚 (异丙酚 )对内毒素刺激小鼠腹腔巨噬细胞TNF α ,IL 6 ,IL 8mRNA表达的影响。方法 :分离巯基乙酸盐诱导的小鼠腹腔巨噬细胞 ,随机分为 7组。A组 :阴性对照组 ;B组 :阳性对照组 ,脂多糖(LPS) 10ng/ml孵育细胞 ;C组 :单用丙泊酚组 ,5 0 0 μg/ml孵育细胞 ;D ,E ,F ,G组 :丙泊酚 +LPS组 ,不同浓度丙泊酚(0 .5 ,5 ,5 0 ,5 0 0 μg/ml)孵育 2h后加入LPS 10ng/ml继续孵育 1～ 4h。用RT PCR法检测巨噬细胞TNF α ,IL 6 ,IL 8mRNA的水平。结果 :在内毒素诱导下 ,腹腔巨噬细胞TNF α ,IL 6 ,IL 8mRNA的水平均显著增高 ;丙泊酚对LPS刺激后TNF α ,IL 6mRNA表达水平的增高有抑制作用 ,并呈浓度依赖性 ;但对LPS刺激下IL 8mRNA表达水平增高的影响无统计学意义。结论 :丙泊酚能在转录水平抑制内毒素刺激下小鼠腹腔巨噬细胞促炎性细胞因子的产生。
Objective: To investigate the effect of propofol on endotoxin-stimulated proinflammatory cytokine mRNA expression of murine celiac macrophages. Methods: Murine celiac macrophages were pretreated with different concentrations of propofol for 2 hours, then incubated with 10 ng/ml LPS for 1-4 hours. RT-PCR was used to measure the mRNA level of tumor necrosis factor alpha, IL-6, IL-8. Results: The levels of TNF-α, IL-6, IL-8 mRNA increased obviously after LPS stimulation but the gene expression of TNF-α,IL-6 was attenuated by propofol in a concentration-dependent manner. However, propofol did not affect IL-8 mRNA expression which had increased after LPS stimulation. Conclusion: Propofol inhibits proinflammatory cytokine gene expression of murine celiac macrophages except IL-8 and may regulate the gene expression of IL-8 through other pathways after the transcription.
出处
《军事医学科学院院刊》
CSCD
北大核心
2004年第3期260-263,共4页
Bulletin of the Academy of Military Medical Sciences
基金
国家自然科学基金资助项目 ( 3 0 10 0 176)
