摘要
丙二烯氧化合酶(allene oxide synthase, AOS)是茉莉酸脂加氧酶合成途径过程中的第一个酶。从冬小麦(Triticum aestivum L. cv. Jinghua No.3)克隆到了该酶的一个全长 cDNA 片段,其开放阅读框长约 1 410 bp,编码一约含470个氨基酸残基的多肽,推测其分子量为 51.9 kD。Southern 分析显示其在基因组中以 3 个拷贝的形式存在。Northern杂交分析表明该基因表达可被外源的茉莉酸诱导,诱导10 h时达到高峰,进一步的RNA 原位杂交表明该基因优先在幼叶中,尤其是在维管束附近的薄壁细胞中表达。同时,原位杂交还显示质膜钙通道的抑制剂 La3+并不能抑制外源茉莉酸诱导该区域 TaAOS 的表达。
Allene oxide synthase (AOS) is the first enzyme in the lipoxygenase pathway which leads to the formation of jasmonic acid (JA). A full length cDNA of TaAOS was cloned in winter wheat (Triticum aestivum L. cv. Jinghua No.3) seedlings. The open reading frame encompassed 1 410 bp encoding a polypeptide of 470 amino acids with calculated mo- lecular mass of 51.9 kD. Southern blot analysis sug- gested there are three copies of the gene in wheat genome. The TaAOS mRNA could be strongly induced by exogenous JA, and the highest level JA was ob- served after a 10 h induction. In situ RNA hybridiza- tion of seedling indicated preferential gene expression in young leaves, especially in the parenchyma cells around the vascular bundles, and the hybridization also showed that exogenous La3+ could not suppress the expression of TaAOS induced by JA.
出处
《植物生理与分子生物学学报》
CAS
CSCD
2004年第4期413-420,共8页
Journal Of Plant Physiology and Molecular Biology
基金
中国科学院知识创新工程项目
国家高技术研究发展计划项目(2001AA222281)资助。~~
