摘要
正常小鼠小肠匀浆经加热、离心、盐析和提取核酸等步骤制备提取物;用单位面积法对各提取物分别进行生物活性鉴定,用紫外法和生化法对提取的核酸进行生化成分的分析。结果表明,从小肠匀浆中提取的DNA和RNA均能提高受照射小鼠的肠腺存活率。并认为其有效成分为核酸类物质。
The intestine homogenate of mice was undergone stepwise pro-cedure of heating, centrifugation. salting out and nucleic acid isolation, and eachfraction was subjected to biological activity assay using the method of crypt coun-ting per unit intestine area in mice. The results showed that the nucleic acid extra-cted from intestine homogenate. either DNA or RNA, was capable of enhancing thecrypt survival in mice after γ-irradiation. These are consistent with our previousresults obtained by using the crude extract of intestine homogenate. Therefore. thecrypt survival enhancing entities in intestine homogenate of mice are identified tobe nucleic acids.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
北大核心
1993年第1期53-57,共5页
Journal of Radiation Research and Radiation Processing
基金
国家自然科学基金
关键词
生物活性
核酸
肠腺
辐照
存活率
Biological activity assay
Efficacious component
Nucleic acid
Irradiation
Intestinal gland recovery
