摘要
提出一种基于胶束增敏测定培氟沙星的荧光分光光度法 ,研究了培氟沙星与十二烷基硫酸钠 (SDS)形成胶束包合物的荧光性质 ,对影响荧光的不同变量和参数进行了研究和优选。试验结果表明 ,在pH 5的BrittonRobinson(BR)缓冲溶液中 ,SDS胶束对培氟沙星有强的增敏作用 ,培氟沙星胶束包合物的激发和发射波长分别为 λex =2 78nm和λem =4 32nm 。其线性范围为 0 0 6～ 1 2 0 μg·mL- 1 ,方法检出限为 0 0 6 μg·mL- 1 ,回收率为 98 5 %～ 10 0 8% ,相对标准偏差为 1 4 %～ 2 3%。该方法已成功用于片剂和血浆中培氟沙星含量的测定 ,其结果令人满意。本文采用同步导数荧光光谱法测定了血浆中的培氟沙星 ,其选择性好 ,灵敏度高 ,可有效地消除血浆中荧光背景的干扰 ,降低了检测限。
A new highly sensitive fluorescence spectrophotometic method for the determination of pefloxacin (PEFX) has been developed based on micella enhancement. The fluorescence characteristic of micelle inclusion complex formed between PEFX and sodium dodecyl sulfate (SDS) was studied. Different variables and parameters affecting the fluorescence were studied and optimized. Experiments show that sodium dodecyl sulfate can enhance greatly the fluorescence signal for pefloxacin in pH 5 Britton Robinson(BR) buffer solution. The fluorimetric method allows the determination of 0.06-1.20 mug(.)ml(-1) of pefloxacin with SDS in aqueous solution with lambda(ex) = 278 nm and lambda(em) = 432 nm, respectively. The detection limit for PEFX is 0.06 mug (.) mL(-1), The recoveries are 98.5%-100.8% and the RSDs; are 1.4%-2.3%. The proposed procedures could be applied successfully to the determination of the pefloxacin drugs in tablets and human plasma with good recovery. In this paper we determined pefloxacin in human plasma by synchronization-derivative fluorescence spectroscopic techniques with good analytical selectivity, high sensitivity, high capability of eliminating blank interference and improving the limit of detection.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2004年第7期855-857,共3页
Spectroscopy and Spectral Analysis
基金
山西省自然科学基金 (2 0 0 1 1 0 1 6)资助课题
