摘要
通过自制的双功能螯合剂 4 ,7 二氯磺基苯 1,10啡啉 2 ,9二羧酸 (BCPDA)标记牛血清白蛋白(BSA)实验 ,对于BCPDA标记蛋白质的条件进行研究。结果表明 :BCPDA在温和的条件下能与蛋白质反应 ,并在一定条件下与铕离子形成稳定的BSA BCPDA Eu3+ 标记物。利用自建的分析方法 ,测定了标记过程的有关参数。并对标记物的某些光学特性进行了研究。
The authors labeled bovine serum albumin (BSA) with a new europium chelator 4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid (BCPDA) which was synthesized by solid phase time-resolved fluorescence immunoassay (TRFIA) technology. The process of BCPDA-labeling-BSA reaction was studied. Using Coomassie brilliant blue method, concentrations of BSA and BCPDA were determined in BCPDA-BSA labeled sample. The labeling reaction condition was studied. Labeling ratio and protein labeling recovery were calculated. BCPDA-BSA-Eu 3+ complex has a very large Stokes shift (270 nm) and can emit very strong fluorescence band at 611.2 nm which is very narrow (10 nm). BCPDA-BSA-Eu 3+ complex exhibits very long fluorescence lifetimes by the experiment demonstration. Also, BCPDA and protein-BCPDA-Eu 3+ complex is relatively stable.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2004年第7期795-798,共4页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金 (30 0 70 71 4 )资助项目
关键词
铕螯合物标记
时间分辨荧光免疫分析
牛血清白蛋白
光学特性
Solid-phase time-resolved fluorimetry
Europium chelator
4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid (BCPDA)
Bovine serum albumin (BSA)
Protein analysis
