摘要
目的 :观察白花前胡中总香豆素组分 (TCP)对小鼠肝药酶活性的影响。方法 :将小鼠随机分组 ,以氯霉素(2 5mg·kg 1 ,ig)为阳性对照 ,观察TCP(5 0 ,10 0 ,2 0 0mg·kg 1 ,ig)分别对戊巴比妥钠 (3 6mg·kg 1 ,ip)和巴比妥钠 (180mg·kg 1 ,ip)催眠作用的影响 ;并用分光光度法测定肝微粒体苯胺羟化酶 (ANH)和氨基比林N 脱甲基酶 (ADM)活性。结果 :TCP呈剂量依赖性地延长戊巴比妥钠的催眠时间 ,但对巴比妥钠的催眠时间无明显影响 ;且TCP能显著降低ANH和ADM活性。结论 :TCP能抑制小鼠肝药酶活性。
Objective:To investigate the effect of the total coumarins from peucedanum praeruptorum Dunn(TCP) on the activity of hepatic drug-metabolizing enzymes in mice. Methods:Mice were randomly grouped. Animals of the blank control,positive control and TCP groups were given each by gastrogavage 5% Tween-80 (20 mL·kg -1),chloromycetin (25 mg·kg -1) and TCP(50,100,200 mg·kg -1)in equal volumes,respectively. An hour later,mice of all groups were given each an intraperitoneal injection of either 36 mg·kg -1 of pentobarbital sodium or 180 mg·kg -1 of barbital sodium. The effects of these 2 hypnotics were kept under observation in all the animals tested and the activities of hepatic microsomal aniline hydroxylase(ANH) and aminopyrine N-demethylase(ADM) determined by spectrophotometry. Results:TCP was shown to dramatically prolong the hypnotic duration of pentobarbital sodium in a dose-dependent manner,but it hardly influenced the hypnotic effect of barbital sodium. TCP in doses of 50 and 100 mg·kg -1 was found to markedly inhibit the activities of ANH and ADM. Conclusion:TCP proved to be capable of inhibiting the activity of hepatic microsomal drug-metabolizing enzymes in mice.
出处
《医药导报》
CAS
2004年第8期522-524,共3页
Herald of Medicine
关键词
白花前胡香豆素
戊巴比妥钠
巴比妥钠
苯胺羟化酶
氨基比林N-脱甲基酶
Coumarins,peucedanum praeruptorum Dunn
Pentobarbital sodium
Barbital sodium
Aniline hydroxylase(ANH)
Aminopyrine N-demethylase(ADM)
