摘要
目的观察赤芍提取物对高脂喂养兔颈总动脉球囊损伤术后血管狭窄有无防止作用。方法采用Clowes方法建立兔颈总动脉球囊损伤模型 ,并给予赤芍提取物。免疫组化染色测定兔颈动脉新生内膜成分、增殖细胞核抗原 (proliferatingcellnuclearantigen ,PCNA)及巨噬细胞表达 ;血管特殊染色观察Ⅰ型胶原增生 ;图像分析系统测定新生内膜面积。结果赤芍提取物可减少PCNA阳性细胞数 ,抑制Ⅰ型胶原增生 ,减轻新生内膜形成。
ObjectiveTo observe the preventive effect of Radix Paeoniae Rubra (RPR) to restenosis after carotid balloon injury in rabbits. MethodsThe rabbit model of carotid balloon injury was established adopting Clowes method, and treated with extract of RPR. Component of new genesic intima and expression of proliferating cell nuclear antigen (PCNA) and macrophage was determined by immunochemical stain. The collagen of type Ⅰwas detected by special staining for blood vessels and the area of new genesic intima was measured by image assay system. ResultsRPR could remarkably decreased the PCNA positive expression and inhibit the proliferation of collagen type Ⅰ and reduce the generating of new intima. ConclusionRPR has significant preventive effect on the restenosis after carotid ballon injury in high fat diet induced atherosclerotic rabbits.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2004年第6期538-540,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
四川省中医管理局专项资助项目 (No .2 0 0 2 55)
关键词
赤芍
高脂喂养
兔
颈总动脉
球囊损伤术
血管狭窄
增殖细胞核抗原
防治措施
restenosis, Radix Paeoniae Rubra, proliferation of intima,proliferating cell nuclear antigen, prevention and treatment
